Lysophosphatidylcholine acyltransferase 1 is involved in the regulation of exosome secretion and uptake in colorectal cancer cells |
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Authors: | HAIZHENG LIU SHAOFEI CHANG |
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Affiliation: | 1.Anorectal Surgery, Yuncheng City Central Hospital, Yuncheng, 044000, China
2 Department of Gastrointestinal and Pancreatic Surgery, Shanxi Provincial People’s Hospital, Taiyuan, 030012, China |
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Abstract: | Lysophosphatidylcholine acyltransferase 1 (LPCAT1) is a phospholipid acyltransferase that promotes
phospholipid synthesis and plasma membrane reconstruction. Exosomes play an important role in tumor metastasis.
The release and uptake of exosomes are key steps of their functions and depend on plasma membrane fusion and
plasma membrane receptors, respectively. The purpose of this study was to explore whether LPCAT1-induced plasma
membrane remodeling would change the secretion and uptake behavior of exosomes in tumor cells. We first
confirmed the abnormally high expression of LPCAT1 in colorectal cancer cells by quantitative real-time PCR (qPCR)
and Western blot analysis. Then, SW620 cells were used as exosome source cells, and SW480 cells were used as
exosome receiver cells. Exosomes from SW620 cells could effectively promote the migration of SW480 cells. When
LPCAT1 expression was reduced via siRNA knockdown in the source cells, the secretion of exosomes was
downregulated, thus weakening the pro-migratory effects of exosomes on target cells. Conversely, when LPCAT1 was
knocked down in target cells, the uptake of exosomes in target cells also decreased sharply. These results undoubtedly
revealed that LPCAT1 is functionally associated with the release and internalization of exosomes in colorectal cancer
cells and could affect the paracrine effects of exosomes, preliminarily extending the classical metabolic function of
LPCAT1 to exosome-related pathways. |
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Keywords: | LPCAT1 Exosomes Colorectal cancer |
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