微操作成像系统中体视显微镜的参数标定

体视显微镜具有工作空间大、实时性高以及不损坏样品表面等优点,己用于微观对象三维立体信息的测量。建立了体视显微镜的成像模型,研究了体视显微镜参数的标定问题。模型包含的方程数目小于参数的数目,需要增加约束条件。结合体视显微镜子光路系统的对称性和微小的差异性,提出了含近似相等条件的体视显微镜参数标定问题的解决方法,试验分析了参数的鲁棒性、相关性和重复性,利用成像模型重构了样板在纵向运动过程中的一组表面。

PARAMETER CALIBRATION OF STEREO LIGHT MICROSCOPE IN MICROMANIPULATION IMAGING SYSTEM

Stereo light microscope which possesses relatively large depth of field, good performance in real-time and no breaking surface of sample, is used in stereo three-dimensional information measurement. An imaging model for stereo light microscope is developed and its parameter calibration is discussed. Because the number of equation in imaging model is less than the number of parameter, constraint must be considered. A new calibration method combining the symmetry and small difference between light subsystems is developed to resolve stereo light microscope calibration with the approximate conditions. The robustness of parameter estimates, parameter correlations and repeatability are analyzed. At last, a series of calibration standards surfaces are reconstructed in its movement.