硫酸类肝素二糖的质谱裂解规律探析

硫酸类肝素结构决定了它与蛋白之间的相互作用,对硫酸类肝素结构的解析,能够进一步阐明结构与功能之间的关系。本实验采用电喷雾离子阱-多级质谱技术(ESI-MSn)在负离子模式(ESI-)下探析了12种硫酸类肝素二糖的裂解规律。实验发现:二糖中亚硫酸根的位置和数量对其在质谱分析中的稳定性起着决定作用,所含亚硫酸根越多越不稳定;N位亚硫酸根比C—2位和C—6位亚硫酸根更容易丢失。此外,二级质谱表明,同分异构体有其特定的裂解规律。该质谱裂解规律有助于硫酸类肝素二糖同分异构体的鉴定,可为多级质谱在硫酸类肝素结构研究中的应用提供理论依据。

Study on the Fragmentation Patterns of Heparan Sulfate Disaccharides by ESI-MS

The structure of heparan sulfate determines its interaction with proteins, therefore the analysis of heparan sulfate can help further clarify structure and function relationships. The characteristic fragmentation pattern of twelve heparan sulfate disaccharide was discussed by electrospray ionization-ion trap mass spectrometry （ESI-MSⁿ） in negative mode. The results show that the position and quantity of sulfate groups in disaccharide determine its stability in MS, moreover, C—2 sulfate and C—6 sulfate in disaccharide are more stable than N-sulfate. MS² revealed that the disaccharide isomers have a characteristic fragment pattern. The results may contribute to identify the disaccharides isomer of heparan sulfate, which can provide theoretical knowleage to identify the structural of heparan sulfate.