进口黑虎虾中霍乱弧菌的鉴定及其挥发性产物分析

目的对进口黑虎虾中分离的菌株F14-2进行种属鉴定,通过顶空气相色谱-质谱联用法(headspace gas chromatography-mass spectrometry,HS-GC-MS)分析分离菌株液态培养代谢的挥发性产物。方法应用VITEK 2 Compact全自动微生物鉴定系统分析菌株的生理生化特征,利用荧光定量PCR特异性扩增检测病原菌,通过顶空气相色谱-质谱联用法分析分离菌株F14-2的代谢的挥发性产物。结果菌株F14-2为革兰氏阴性菌,生理生化特征与霍乱弧菌(Vibrio cholerae)的相似性为98%,荧光定量PCR检测和血清凝集试验进一步确认为非O1非O139群霍乱弧菌,菌株F14-2在营养肉汤(nutrient broth)中代谢的挥发性产物主要有乙醇、异戊醇、乙酸和2-乙基己醇。结论进口黑虎虾中分离的菌株F14-2鉴定为霍乱弧菌,该菌产生的挥发性产物为霍乱弧菌的鉴定提供参考。

Identification of Vibrio cholerae and analysis of its volatile compounds in imported Penaeus monodon

Objective To identify bacterial strain F14-2 isolated from the imported Penaeus monodon and analyze its volatile compounds (VCs) of liquid-state fermentation by headspace gas chromatography-mass spectrometry (HS-GC-MS). Methods The physiological and biochemical characteristics were elucidated by VITEK 2 compact automated microbiology system. Strain F14-2 was detected by real-time PCR method. Volatile compounds (VCs) from F14-2 cultured with nutrient broth were analyzed by headspace gas chromatography-mass spectrometry (HS-GC-MS). Results Strain F14-2 was identified as Gram-negative and exhibited the highest levels of 98% probability to be Vibrio cholerae based on the conventional physiological test. It was confirmed as non-O1 and non-O139 Vibrio cholera by real-time PCR and serum agglutination test. The main VCs produced by F14-2 in nutrient broth were detected to be ethanol, 3-methyl-1-butanol, acetic acid and 2-ethylhexanol. Conclusion Bacterial strain F14-2 isolated from the imported Penaeus monodon is identified as Vibrio cholera. The VCs produced by F14-2 will provide basis for the identification of Vibrio cholera.