| 大黄鱼中哈维氏弧菌毒力及耐药特性分析 |
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| 引用本文： | 吴立婷,廖金轩,庞茂达,包红朵,周 艳,孙利厂,王 冉,张 辉.大黄鱼中哈维氏弧菌毒力及耐药特性分析[J].食品安全质量检测技术,2019,10(8):2111-2119. |
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| 作者姓名： | 吴立婷 廖金轩 庞茂达 包红朵 周 艳 孙利厂 王 冉 张 辉 |
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| 作者单位： | 江苏省食品质量安全重点实验室-省部共建国家重点实验室培育基地江苏省农业科学院农产品质量安全与营养研究所;扬州大学生物科学与技术学院 |
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| 基金项目： | 国家自然科学基金项目(31671955)、江苏省农业科技自主创新资金项目[cx(16)1028] |
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| 摘 要： | 目的分析鉴定海产养殖中主要病原哈维氏弧菌的毒力及耐药特性。方法采集来自于网箱养殖区病死大黄鱼,分离幵鉴定哈维氏弧菌,利用微量肉汤稀释法分析分离株对13种抗生素的敏感特性,PCR鉴定耐药基因(tetA、tetB、ermA、ermB、ermC、mecA、aac(6’)-Ib、oqxA、sul1、sul2、mcr-1)及其毒力基因。结果从病料中分离幵鉴定出54株哈维氏弧菌,耐药分析表明对硫酸粘杆菌素、庆大霉素、四环素、氨苄西林、复方新诺明和阿莫西林均耐药, 96.2%的分离株对头孢噻呋, 92.6%的分离株对链霉素、阿米卡星表现为耐药, 88.9%的分离株对恩诺沙星, 87.1%的分离株对卡那霉素呈现高度耐受特性。所有分离株同时对9种抗生素显示100%耐药,同时耐受10种抗生素的菌株有94.4%,同时对11和12种抗生素耐受的菌株分别达到85.2%和64.8%,对13种抗生素均产生耐药的菌株达到37.0%(20/54)。耐药基因分析表明四环素类tetA为主要耐药基因,检出率高达81.5%;氨基糖苷类aac(6’)-Ib,β-内酰胺类mecA,磺胺类sul1、sul2,喹诺酮类oqxA和大环内酯类ermA、ermC分别检出4～7株。毒力基因分析表明toxR携带率高达75.9%。结论本研究分析了海产养殖中主要病原哈维氏弧菌感染状况,其耐药严重且普遍携带毒力基因,表明哈维氏弧菌耐药和毒力对海产养殖造成重要威胁。同时本研究为弧菌病的有效防治提供参考依据。
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| 关 键 词： | 哈维氏弧菌 耐药特征 耐药基因 毒力基因 |
| 收稿时间： | 2019/2/19 0:00:00 |
| 修稿时间： | 2019/4/3 0:00:00 |
Virulence and antimicrobial resistance of Vibrio harveyi in large yellow croaker |
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| WU Li-Ting,LIAO Jin-Xuan,PANG Mao-D,BAO Hong-Duo,ZHOU Yan,SUN Li-Chang,WANG Ran and ZHANG Hui.Virulence and antimicrobial resistance of Vibrio harveyi in large yellow croaker[J].Food Safety and Quality Detection Technology,2019,10(8):2111-2119. |
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| Authors: | WU Li-Ting LIAO Jin-Xuan PANG Mao-D BAO Hong-Duo ZHOU Yan SUN Li-Chang WANG Ran and ZHANG Hui |
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| Affiliation: | Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Institute of Food Safe and Nutrition, Jiangsu Academy of Agricultural Sciences,College of Biological Sciences and Technology, Yangzhou University,Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Institute of Food Safe and Nutrition, Jiangsu Academy of Agricultural Sciences,Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Institute of Food Safe and Nutrition, Jiangsu Academy of Agricultural Sciences,Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Institute of Food Safe and Nutrition, Jiangsu Academy of Agricultural Sciences,Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Institute of Food Safe and Nutrition, Jiangsu Academy of Agricultural Sciences,Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Institute of Food Safe and Nutrition, Jiangsu Academy of Agricultural Sciences and Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Institute of Food Safe and Nutrition, Jiangsu Academy of Agricultural Sciences |
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| Abstract: | |
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| Keywords: | Vibrio harveyi antimicrobial resistance resistance gene virulence gene |
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