| 动态显色法鲎试验用于果汁细菌内毒素活性检测及干扰分析 |
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| 引用本文： | 白 淼,张 灿,张明露,徐梦瑶,史 云,王莉莉,董德荣.动态显色法鲎试验用于果汁细菌内毒素活性检测及干扰分析[J].食品安全质量检测技术,2019,10(10):3192-3196. |
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| 作者姓名： | 白 淼 张 灿 张明露 徐梦瑶 史 云 王莉莉 董德荣 |
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| 作者单位： | 中国人民解放军疾病预防控制中心;北京工商大学环境科学与工程系 |
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| 基金项目： | 国家重点研发计划(2016YFD0401202) |
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| 摘 要： | 目的建立一种定量检测果汁细菌内毒素活性的方法,准确评估果汁潜在的内毒素污染风险。方法采用动态显色法东方鲎(Tachypleus amebocyte lysate, TAL)试验对常见的4种果汁(橙汁、桃汁、苹果汁和葡萄汁)细菌内毒素活性进行检测,对果汁样品中的干扰因素进行识别与排除,最后使用抗增液排除β-葡聚糖等类似物质带来的假阳性结果。结果市售果汁(n=36)的细菌内毒素活性范围为0.2～209.5 EU/mL,自榨果汁(n=12)的细菌内毒素活性范围为0.4～4.2EU/mL,加标回收率范围均在50%～200%之间,符合药典要求。结论动态显色法TAL试验适用于常见的4种果汁(橙汁、桃汁、苹果汁和葡萄汁)细菌内毒素活性的检测,为果汁的健康风险评价和卫生监管提供参考。
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| 关 键 词： | 果汁 内毒素活性 动态显色法 鲎试验 微生物污染 |
| 收稿时间： | 2019/2/19 0:00:00 |
| 修稿时间： | 2019/4/11 0:00:00 |
Quantitative detection and influence factors of endotoxin activities in juice products using by Tachypleus amebocyte lysate assay of kinetic chromogenic analysis |
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| BAI Miao,ZHANG Can,ZHANG Ming-Lu,XU Meng-Yao,SHI Yun,WANG Li-Li and DONG De-Rong.Quantitative detection and influence factors of endotoxin activities in juice products using by Tachypleus amebocyte lysate assay of kinetic chromogenic analysis[J].Food Safety and Quality Detection Technology,2019,10(10):3192-3196. |
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| Authors: | BAI Miao ZHANG Can ZHANG Ming-Lu XU Meng-Yao SHI Yun WANG Li-Li and DONG De-Rong |
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| Affiliation: | Center for Disease Control and Prevention of Chinese PLA,Center for Disease Control and Prevention of Chinese PLA,Department of Environmental Science and Engineering, Beijing Technology and Business University,Department of Environmental Science and Engineering, Beijing Technology and Business University,Center for Disease Control and Prevention of Chinese PLA,Center for Disease Control and Prevention of Chinese PLA and Department of Environmental Science and Engineering, Beijing Technology and Business University |
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| Abstract: | |
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| Keywords: | juice endotoxin activities kinetic chromogenic assay Tachypleus amebocyte lysate (TAL) assay microbiological contamination |
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