气相色谱法测定调和油中18种脂肪酸

目的建立气相色谱法测定调和油中18种脂肪酸的方法。方法样品经过氢氧化钾甲醇溶液甲酯化,采用毛细管色谱柱(100 mm×0.25 mm, 0.2μm)和程序升温模式进行测定,检测器温度为280℃。结果 18种脂肪酸的线性关系良好(r>0.999),检出限为0.0005~0.009mg/kg,回收率为78.9%~99.1%,相对标准偏差(relative standard deviation, RSD)为0.768%~8.236%。结论该方法快速、简单、回收率和精密度良好,可用于调和油中脂肪酸的的检测。

Determination of 18 fatty acids in mixed oil by gas chromatography

A gas chromatographic method for the determination of eighteen fatty acids in blending oil was established. The sample was esterified with potassium hydroxide methanol solution, and the capillary column (100 mm 0.25 mm, 0.2 m) and temperature programmed mode were used. The detector was hydrogen flame ion detector at 280 °C. The results showed that the detection limit of 18 fatty acids was 0.0005-0.009 mg / KG, 78.9%-99.1% , RSD was 0.768%-8.236% . The method was rapid, simple, with good recovery and precision, and could be used for the determination of fatty acids in oil.