基于稳定同位素技术的4种食源致病菌蛋白质组研究进展

目前微生物蛋白质组差异比较研究的最大困难在于无法获得稳定的内参照。而稳定同位素与其同位素存在质量差异,又不具有放射性,因此是分子差异比较研究的最理想的内参照,其作用是校正实验过程中存在的误差,保证实验结果的准确性。稳定同位素技术结合质谱分析的目标是:建立微生物(主要是致病菌)的稳定同位素体内标记技术并将标记产物作为内标用于微生物蛋白质组表达差异的研究,根据获得的差异信息为特殊处理条件下微生物的检测提供解决办法。本文从稳定同位素国内外研究进展、传统经典蛋白质组研究方法存在的不足和常见的4种食源性致病菌,包括沙门氏菌、单核增生李斯特氏菌、霍乱弧菌和副溶血弧菌的蛋白质组研究,并对副溶血性弧菌的蛋白质组研究作详细阐述。最后阐述稳定同位素标记的蛋白质组定量方法存在的一些挑战和今后的努力改进和发展的方向。

Proteomics analysis of 4 kinds of foodborne pathogenic bacteria based on stable isotope technique

ABSTRACT: Lack of stable internal control is a serious obstacle in comparative proteomics research. Stable isotope, considered as most ideal internal control in terms of quality difference compared with its isotopes and non-radioactivity, plays a crucial role in minimizing error and ascertaining result accuracy. This article is related to progress in proteomic research globally and disadvantages in traditional proteomic research as well as the proteomic research in four common foodborne bacteria including Salmonella, Listeria monocytogenes, Vibrio cholera and V. parahaemolyticus (in detail). The aim of stable isotope labeling combined with mass spectrometry is to reveal the differences of proteomic expressions via interior control based on stable isotope labeling approaches, and to provide detection methods for special-treated microorganisms. Finally, challenges in proteomic quantitation were elaborated and improvements as well as development trends of proteomics research were present.