基于微量元素多元统计和微观形态分析的花椒叶产地及品种判别

为解决不同产地、品种花椒叶混售的问题,采用湿法消解结合电感耦合等离子体发射光谱(ICP-OES)技术同步检测不同产地、品种的12个样品中的Ba、Ce、Ho等10种微量元素。利用主成分分析与聚类分析对数据进行研究,结果表明：2个主成分贡献率达到70%,样品可显著聚类为2类,伏椒叶与油椒叶的元素含量差异显著,不同产地的花椒叶也能明显区分。基于扫描电镜(Scanning electron microscope , SEM)的微观形态分析佐证了多元统计分析的结论,伏椒叶与油椒叶结构差异显著,不同产地的花椒叶表现出的结构差异性或相似性都符合统计分析的分类结果。研究能够有效的判别不同产地、品种的花椒叶。

Origin and cultivar identification of Zanthoxylum bungeanum leaves based on multivariate statistics of trace elements and micromorphological analysis

In order to solve the problem of mixed sale of Zanthoxylum bungeanum leaves from different producing areas and varieties, wet digestion combined with inductively coupled plasma atomic emission spectrometry (ICP-OES) was used to simultaneously detect 10 trace elements (BA, CE, Ho) in 12 samples from different producing areas and varieties. Principal component analysis and cluster analysis were used to study the data. Expression of results is that the contribution rate of the two principal components reached 70%, and the samples could be significantly clustered into two groups. There were significant differences in element contents between leaves of Fujiao and Youjiao. The leaves of Zanthoxylum bungeanum from different areas can also be distinguished. The conclusion of multivariate statistical analysis is supported by the micro morphological analysis based on SEM. There was significant difference in structure between leaves of Fujiao and Youjiao. The structural differences or similarities of Zanthoxylum bungeanum leaves from different habitats were consistent with the classification results of statistical analysis. The study can effectively distinguish the leaves of Zanthoxylum bungeanum from different producing areas and varieties.