| 牛乳中微生物酶的分离及危害研究 |
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| 引用本文： | 生庆海,张爱霞,刘晓东,李志钧.牛乳中微生物酶的分离及危害研究[J].中国乳品工业,2005,33(3):22-24. |
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| 作者姓名： | 生庆海 张爱霞 刘晓东 李志钧 |
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| 作者单位： | 1. 石家庄三鹿集团股份有限公司,河北,石家庄,050071
2. 河北省农林科学院经济作物研究所,河北,石家庄,050051 |
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| 摘 要： | 牛乳中存在的耐热芽孢杆菌和某些假单胞菌能够分泌蛋白水解酶类。采用(NH4)2SO4沉淀，高速离心的方法对原料乳中微生物产酶进行分离提取，在366m波长处比色，结果证实有一定的酶活存在。通过分析酶与UHT灭菌乳质量的关系，发现酶能使乳蛋白发生水解，酸度增大，并出现沉淀现象。
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| 关 键 词： | 微生物酶 分离 活性测定 影响 |
| 文章编号： | 1001-2230(2005)03-0022-03 |
Research on the separation and hazard of microbial proteases in milk |
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| SHENG Qinghai,ZHANG Aixia,LIU Xiaodong,LI Zhijun.Research on the separation and hazard of microbial proteases in milk[J].China Dairy Industry,2005,33(3):22-24. |
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| Authors: | SHENG Qinghai ZHANG Aixia LIU Xiaodong LI Zhijun |
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| Affiliation: | SHENG Qing-hai1,ZHANG Ai-xia1,LIU Xiao-dong2,LI Zhi-jun1, |
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| Abstract: | Heat-resistant spore bacillus and some Pseudomonas in milk can secrete proteases. The activity of proteases can be measured in 366nm wavelength, which was seperated from microorganism by (NH4)2SO4 deposition and high speed centrifuge. The relationship between proteases and UHT milk was analyzed. And the proteases can hydrolyze the milk protein, resulting in acidity increasing and deposition. |
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| Keywords: | microbial proteases separation activity determination influence |
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