高效液相色谱法快速测定乳粉中叶黄素的质量分数

建立一种测定配方奶粉中叶黄素质量分数的高效液相色谱方法。采用Diamonsil C18色谱柱(5μm,250 mm×4.6 mm),流动相为甲醇-水(97∶3),流速1.0 mL/min,检测波长445 nm,采用空白基质加标实验绘制标准曲线,该方法在0.5～100 mg/L的质量浓度范围内线性关系良好(R=0.9987),加标水平为0.50,1.00,5.00μg/g时,回收率分别介于96.8%～98.4%之间,相对标准偏差介于4.27%～6.73%之间,该方法检测限低于0.10μg/g。该方法操作简便,灵敏度高、准确度高、重现性好,适合于配方奶粉中叶黄素的测定。

Determination of lutein in milk powder by HPLC

A HPLC method for determination of lutein in milk powder.The analysis was carried on a Diamonsil C18(5 μm,250 mm×4.6 mm)column,the mobile phase was methanol-water(97∶3),the flow-rate was set at 1.0 mL·min-1,chromatographic runs were montitored at 445 nm.When analyzing samples under the same conditions,draw matrix calibration curves.The results showed that the linearity of the method was good between 0.5～100 mg/L(R=0.9987).The recoveries were in the range from 96.8% to 98.4% at the spiked levels of 0.50,1.00,5.00 μg/g,and the relative standard deviation were between 4.27%～6.73% in different concentration level.The limit of detection was 0.10 μg/g.The method is simple,sensitive,accurate and precise for determination of lutein in milk powder.