| 产高黏性黄原胶基因工程菌株的构建 |
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| 引用本文： | 张晓元,陈晓燕,颜震,王桂兰,朱希强,郭学平,凌沛学.产高黏性黄原胶基因工程菌株的构建[J].食品与药品,2011(3):83-85. |
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| 作者姓名： | 张晓元 陈晓燕 颜震 王桂兰 朱希强 郭学平 凌沛学 |
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| 作者单位： | 山东省生物药物研究院; |
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| 摘 要： | 目的获得产高黏度黄原胶基因工程菌株。方法利用PCR技术,以野油菜黄单胞菌X58基因组为模板扩增得到产胶基因gumBC,通过纯化、内切酶酶切、连接和接合,构建了含gumBC基因表达载体(pBBR-gumBC)的重组菌株X58-BC。结果经10 L发酵罐实验证实,gumBC过量表达,黄原胶黏度值(Brookfield s05,60 r/min)提高了62%,剪切性能值提高了57%。结论成功构建了产高黏度黄原胶基因工程菌株。
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| 关 键 词： | 野油菜黄单胞菌 基因改造 高黏性 |
Construction of Recombinant Strain Producing High Viscosity Xanthan Gum |
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| ZHANG Xiao-yuan,CHEN Xiao-yan,YAN Zhen,WANG Gui-lan,ZHU Xi-qiang,GUO Xue-ping,LING Pei-xue.Construction of Recombinant Strain Producing High Viscosity Xanthan Gum[J].Food and Drug,2011(3):83-85. |
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| Authors: | ZHANG Xiao-yuan CHEN Xiao-yan YAN Zhen WANG Gui-lan ZHU Xi-qiang GUO Xue-ping LING Pei-xue |
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| Affiliation: | ZHANG Xiao-yuan,CHEN Xiao-yan,YAN Zhen,WANG Gui-lan,ZHU Xi-qiang,GUO Xue-ping,LING Pei-xue(Institute of Biopharmaceuticals of Shandong Province,Jinan 250101,China) |
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| Abstract: | Objective To obtain the recombinant strain producing high viscosity xanthan gum.Methods GumBC gene from Xanthomonas campestris X58 was cloned by PCR.The recombinant expression plasmid pBBR-gumBC was constructed by recombinant DNA technique and then conjugated into Xanthomonas campestris X58.Results The viscosity value(Brookfield s05 60r/min) and shear value of xanthan gum produced by X58-gumBC in 10L stirred fermentor after overexpression of gumBC were increased by 62 % and 57 % respectively.Conclusion The ... |
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| Keywords: | Xanthomonas campestris recombinant strain high viscosity |
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