富硒玉米肽对四氯化碳致小鼠急性肝损伤的保护作用

研究富硒玉米肽对四氯化碳(Carbon tetrachloride,CCl4)诱导的小鼠肝损伤的保护作用。将健康的雄性昆明小鼠70只,随机分成7组:正常对照组、CCl4模型组、富硒玉米肽低、中、高剂量组(100、200、400mg/kg·bw)、普通玉米肽组(200 mg/kg·bw)、水飞蓟素阳性对照组(50 mg/kg·bw),每组10只。测定比较血清中谷丙转氨酶(ALT)活力、谷胱甘肽(GSH)、三酰甘油(TG)含量,同时测定肝组织中丙二醛(MDA)、谷胱甘肽(GSH)的含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力,肝脏指数,并观察肝组织病理学变化。结果显示富硒玉米肽低、中、高剂量组均能极显著抑制CCl4致肝损伤小鼠血清ALT活性和TG含量的升高、GSH含量的降低(P0.01);均能极显著提高肝组织中GSH含量和SOD、GSH-Px活力,并降低肝组织中MDA的含量(P0.01),极显著降低由于肝损伤引起的肝脏肿大(P0.01),改善肝组织损伤程度,并存在剂量效应关系。富硒玉米肽高剂量组的护肝效果与50 mg/kg·bw的水飞蓟素相当,相同剂量的富硒玉米肽的保肝作用极显著优于普通玉米肽(P0.01)。结果表明富硒玉米肽对CCl4所致小鼠急性肝损伤有显著的保护作用,且与普通玉米肽相比,富硒玉米肽的有效保肝剂量显著降低。

Hepatoprotective effects of the Selenium-enriched Corn Peptides against Carbon Tetrachloride-induced Liver Injury in Mice

The hepatoprotective effects of the selenium-enriched corn peptides were evaluated against carbon tetrachloride (CCl4)-induced liver injury in mice. Seventy healthy Kunming male mice were randomized into 7 groups with 10 per-group: normal control group, CCl4-injured model group, low dose , medium dose and high dose (100, 200 and 400 mg/kg?bw), selenium-enriched corn peptides treatment group, corn peptides (200mg/kg?bw) treatment group, positive control group-silymarin (50 mg/kg?bw) treatment group. The activities of alanine aminotransferase (ALT), glutathione (GSH) level, triglyceride (TG) level in serum, the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), malondialdehyde (MDA) level, glutathione (GSH) level in liver and liver index were determined. The morphology of liver tissues was also examined. Results showed that selenium-enriched corn peptides could significantly decrease ALT activities, TG level in serum, MDA level in liver (P<0.01) and the liver enlargement, the selenium-enriched corn peptides could significantly increase GSH level, SOD activity, GSH-Px activity in liver (P<0.01) in a dose-dependent manner. The biochemical results were supplemented by histopathological examination of liver sections. The hepatoprotective effect of selenium-enriched corn peptides was significantly better than the corn peptides under the same dose (P<0.01).When the concentration of selenium-enriched corn peptides reached to 400 mg/kg?bw, its effect was approximate to silymarin (50 mg/kg?bw) .In conclusion, this study demonstrated that selenium-enriched corn peptides have an significant protective effect against CCl4-induced liver damage, the dose of selenium-enriched corn peptides with the potent hepatoprotective effect was significantly less than that of the corn peptides.