| 卵白蛋白抗氧化肽分离与纯化 |
| |
| 引用本文： | 沈勇根,徐明生,尹忠平,吴少福.卵白蛋白抗氧化肽分离与纯化[J].中国食品学报,2011,11(8). |
| |
| 作者姓名： | 沈勇根 徐明生 尹忠平 吴少福 |
| |
| 作者单位： | 江西农业大学食品科学与工程学院 江西省教育厅天然产物研究与开发重点实验室 南昌330045 |
| |
| 摘 要： | 目的:从卵白蛋白酶解液中分离抗氧化肽.方法:通过超滤、离子色谱和反相高效液相色谱法从卵白蛋白酶解液中分离制备抗氧化肽,用电喷雾质谱肽的结构进行表征.结果:分离纯化获得抗氧化能力较强的3个肽:OPQ3-1、OPQ3-2和OPQ3-4,分别是源于鸡蛋卵白蛋白第115～117氨基酸残基(Pro-Glu-Tyr),分子质量408.14 Da;第245～248氨基酸残基(Leu-Pro-Asp-Glu),分子质量473.19 Da;第148～150氨基酸残基(Trp-Val-Glu),分子质量433.18 Da.结论:卵白蛋白经胃蛋白酶水解获得抗氧化多肽.
|
| 关 键 词： | 卵白蛋白 酶解 抗氧化肽 分离 |
Isolation and Purification of Antioxidant Peptide from Ovalbumin |
| |
| Shen Yonggen,Xu Mingsheng,Yin Zhongping,Wu Shaofu.Isolation and Purification of Antioxidant Peptide from Ovalbumin[J].Journal of Chinese Institute of Food Science and Technology,2011,11(8). |
| |
| Authors: | Shen Yonggen Xu Mingsheng Yin Zhongping Wu Shaofu |
| |
| Affiliation: | Shen Yonggen Xu Mingsheng* Yin Zhongping Wu Shaofu (College of Food Sci-Eng,Jiangxi Agriculture University,Key Laboratory of Jiangxi Provincial Department of Education in Natural Product Research and Development,Nanchang 330045) |
| |
| Abstract: | Objective:Isolation antioxidative peptide from ovalbumin by enzymatic hydrolysis. Method:Antioxidative peptides in the hydrolysates were purified by ultrafiltration(UF) membrane system, Q-Sepharose FF ion-exchang chromatography and reverse phase high-performance liquid chromatography(RP-HPLC). Their structure was further identified by electrospray ionization tandem mass spectrometry (ESI-MS/MS). Result:Three peptides with strong antioxidative activities were purified from the hydrolysates. Their structure w... |
| |
| Keywords: | ovalbumin enzymatic hydrolysis antioxidative peptide isolation |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
