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阻抗法快速分析牛奶中的菌落总数
引用本文:张爱萍,孟瑞锋,侯式娟,唐佳妮,刘东红.阻抗法快速分析牛奶中的菌落总数[J].中国食品学报,2012,12(2):158-164.
作者姓名:张爱萍  孟瑞锋  侯式娟  唐佳妮  刘东红
作者单位:1. 浙江大学生物系统工程与食品科学学院 杭州 310029
2. 烟台大学化学生物理工学院 山东烟台264005
基金项目:十一五国家科技支撑课题
摘    要:以电导、总阻抗、双电层电容、培养介质电容、阻抗角作为检测参数,比较含初始菌数不同的阻抗测试管的阻抗变化率曲线,结果阻抗角不适于作为阻抗法快速分析牛奶中菌落总数的检测参数。将检出时间DT值和平板计数法得到的菌落总数的对数值lgC用SAS做线性回归,结果电导、总阻抗、双电层电容之间存在线性相关。用Δ=lg(C0/CSPC)=lg(C0)-lg(CSPC)值分析阻抗法的检测误差,结果由电导、总阻抗作为检测参数得到的菌落总数值符合实际情况。用阻抗法快速分析牛奶中的菌落总数时,分析FPR、FNR随DTTH的变化趋势,DTTH值分别选440min(电导和总阻抗)和410min(双电层电容)。

关 键 词:牛奶  阻抗  菌落总数

Rapid Detection of Total Bacteria Count in Milk by the Impedance Method
Zhang Aiping , Meng Ruifeng , Hou Shijuan , Tang Jiani , Liu Donghong.Rapid Detection of Total Bacteria Count in Milk by the Impedance Method[J].Journal of Chinese Institute of Food Science and Technology,2012,12(2):158-164.
Authors:Zhang Aiping  Meng Ruifeng  Hou Shijuan  Tang Jiani  Liu Donghong
Affiliation:1 (1College of Biosystem Engineering and Food Science,Zhejiang University,Hangzhou 310029 2Chemistry & Biology College,Yantai University,Yantai 264005,Shandong)
Abstract:Took the conductance,the total impedance,the double-layer capacitance,the medium capacitance,the impedance angle as the examination parameter,compared and analyzed curves of change rate of these impedance parameters,obtaining that the impedance angle not be suitable for the analysis of the total bacteria count of milk.When the examination parameter was the conductance,the total impedance,or the double-layer capacitance,there was linear correlation between the detection time(DT) and the log10 initial bacterial counts.Analyzed the examination error by the formula Δ=lg(C0/CSPC)=lg(C0)-lg(CSPC),obtaining the conductance,the total impedance be more suitable than the double-layer capacitance.Finally,compared the change tendency of FPR,FNR along with the DTTH,obtaining the suitable value of DTTH can be 440min(the conductance and the total impedance as parameter),410min(the double-layer capacitance as parameter).
Keywords:milk  impedance  the total bacteria count
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