角蛋白酶对羊毛蛋白酶防毡缩整理的促进作用

采用Bacillus subtilis产角蛋白酶与蛋白酶-浴法处理羊毛,研究处理后羊毛织物的毡缩率、减量率、强力、润湿性及纤维直径等指标的变化,测试羊毛和羊毛角蛋白水解液中蛋白多肽的释放速率.结合羊毛的溴Allwǒrden现象,通过SEM观察,考察2种酶协同对蛋白质的降解作用、对羊毛防毡缩性能的影响和对羊毛鳞片的去除效...

Promoting effect of keratinase in wool anti-felting finishing with protease

A novel keratinase from Bacillus subtilis and protease were used to treat wool in one-bath method, and the change in felting shrinkage, weight loss, strength, wettability, and fibre diameter of the treated wool fabric was tested, along with polypeptide release rate in the hydrolyte solution of enzyme-treated wool and keratin of wool. Combining with wool bromine Allwörden phenomenon, SEM was use to investigate the degradation effect of protein, wool anti-felting property and the removal of wool scales affected by synergistic effect of the two enzymes. Compared with specimens only treated by keratinase or protease, the felting shrinkage of wool fabric treated by keratinase and protease in one-bath dropped significantly, and reached machine washable requirements, but the fabric weight loss was larger, strength reduced, and fibre diameter decreased. UV spectrum of the hydrolyte solution showed that one- bath process could effectively improve the polypeptide release rate, and protease had no obvious effect on the structural stability of keratinase. Wool bromine Allwörden phenomena and wettability test results showed that the lipid above scales had been basically removed. The result of SEM showed wool scales had basically been stripped. All of these results showed that keratinase could promote protease to degrade wool scales, and the synergistic effect of the two enzymes was able to remove wool scales effectively.