丝素蛋白/聚左旋乳酸纳米纤维纱线肌腱补片的制备及其性能

针对用于纺织肌腱补片的纯微米纱线结构生物活性低、免疫原性强和不可降解的缺陷,从补片材质和结构优化角度出发,构建了几种可生物吸收的纳米结构肌腱补片,以自制的丝素蛋白(SF)/聚左旋乳酸(PLLA)纳米纤维纱线为纬纱,以传统PLLA微米纤维纱线为经纱,经机织工艺加工成形。系统研究了SF和PLLA组分配比对补片形态结构、理化性能以及生物性能的影响。结果表明:补片均在约16.4°出现了结晶衍射峰,且随着SF占比的增加,补片的结晶度和力学性能均逐渐降低,但其断裂载荷均在100 N以上,可满足实际应用需求,且补片的细胞黏附和增殖能力均随SF质量分数的增加逐渐增强。

Preparation and properties of silk fibroin/poly(l-lactic acid) nanofiber yarns-based tendon patches

Textile tendon patches made of pure micro yarn structure has low biological activity, high immunogenicity and is non-biodegradable. Several nano-structured tendon patches with great bioabsorbability were fabricated by weaving with optimized patch material and structure. The weft yarn was constructed with the several different types of silk fibroin (SF)/poly(l-lactic acid) (PLLA) nanofiber yarns, the warp yarn was all composed of traditional PLLA microfiber yarns, and the two sets of yarns were interweaved into patches through the weaving process. The effects of the SF and PLLA mass ratio of nanofiber yarns on the morphology, physicochemical and biological properties of such patches were systematically investigated. The results showed that the crystal diffraction appeared at about 16.4°, and crystallinity and mechanical properties of the patches decreased gradually with the increase of SF content. The breaking loads of all tendon patches were found to be higher than 100 N, meeting the requirement of practical application. The biological tests showed that cell adhesion and proliferation ability of the patches gradually improved with the increase of SF content.