Characterization of host response to bruchids (Callosobruchus chinensis and C. maculatus) in 39 genotypes belongs to 12 Cajanus spp. And assessment of molecular diversity inter se |
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| Affiliation: | 1. Plant Biotechnology Laboratory, Department of Biotechnology and Bioinformatics, Sambalpur University, Jyoti Vihar, 768019, Odisha, India;2. Plant Molecular Breeding and Functional Genomics Laboratory, Department of Biotechnology, Central University of Rajasthan, NH-8, Bandarsindri, 305817, Kishangarh, Ajmer, Rajasthan, India;3. Department of Bioscience and Bioinformatics, Khallikote University, Berhampur, 760001, Odisha, India;1. Department of Life Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago;2. Department of Food Production, The University of the West Indies, St. Augustine, Trinidad and Tobago;1. Crop Research Institute, Drnovska 507, Prague 6, Czech Republic;2. Department of Zoology, Faculty of Science, Charles University, Vinicna 7, Prague 2, Czech Republic;3. National Institute of Public Health, Srobarova 48, Prague 10, Czech Republic;1. Department of Biological Sciences, Arkansas State University, Jonesboro, AR, 72467, United States;2. Arkansas Agricultural Experiment Station, Arkansas State University Research Unit, Jonesboro, AR, 72467, United States;3. Department of Food Science, University of Arkansas & Division of Agriculture, Fayetteville, AR, 72704, United States;1. Laboratory of Entomology and Agricultural Zoology, Department of Agriculture, Crop Production and Rural Environment, University of Thessaly, Phytokou Str., Volos, 38446, Greece;2. BASF Crop Protection, BASF Plant Science, Speyerer Straße 2, D-67117, Limburgerhof, Germany;3. BASF Hellas, Paradissou 2 and Kifissias, 151 25, Maroussi, Athens, Greece;4. BASF Corporation, 26 Davis Drive, Research Triangle Park, 27709-3528, NC, USA;1. IRTA, Ctra. Cabrils km 2, E-08348, Cabrils, Barcelona, Spain;2. IRTA, Finca Camps i Armet, E-17121, Monells, Girona, Spain |
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| Abstract: | Thirty nine genotypes belong to 12 Cajanus species were characterized for host response to bruchids (C. chinensis and C. maculatus) using no-choice based in vivo and in vitro antibiosis assay, and these assays revealed seven genotypes of primary gene pool (C. cajan) were more susceptible to bruchid infestation as compared to the genotypes of secondary and tertiary gene pools. Among all the genotypes, C. cajanifolius acc. ICPW-31 showed higher degree of resistance to both the bruchids. The molecular diversity using CDDP and SCoT markers showed wide range of genetic variations among the 12 species of Cajanus, and was supported by estimates of Nei's genetic distance and fixation index (FST) based haplotype matrix. The genetic structure showed clustering of 39 genotypes into eight distinct groups (K = 8) on the basis of their allelic composition, and among them C. cajanifolius acc. ICPW 31 and ICPW 30 showed close affinity with the cultivars of C. cajan by sharing several alleles. AMOVA analyses showed the existence of higher extent of genetic variation both at the genotype (37%) and species (63%) level in the genus Cajanus. The dendrogram and the principal coordinate analysis (PCoA) placed the 39 genotypes into six major clusters at par with their sectional classification. MCheza and ARLEQUIN based outlier analysis revealed 13 common loci under balancing selection, which are supposed to be involved in maintenance of genetic polymorphism either at species or genotype level, and are assigned to putative gene families such as KNOX, WRKY, ERF, MYB, ABP1 and MADS. The in vivo and in vitro bruchid assay vis-à-vis the DNA marker based molecular diversity analysis affirmed the possible use of C. cajanifolius acc. ICPW-31 as donor genotype for the introgression of bruchid resistance allele(s) into cultivated genetic background. |
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| Keywords: | Molecular diversity Bruchid resistance Outlier analysis AMOVA |
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