| 香蕉低聚糖的分离纯化及理化性质研究 |
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| 引用本文： | 王娟,程燕锋,苏锦明,杨公明.香蕉低聚糖的分离纯化及理化性质研究[J].食品与发酵工业,2008,34(12). |
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| 作者姓名： | 王娟 程燕锋 苏锦明 杨公明 |
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| 作者单位： | 1. 华南农业大学食品学院,广东,广州,510642;华南理工大学轻工与食品学院,广东,广州,510641
2. 华南农业大学食品学院,广东,广州,510642 |
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| 基金项目： | 国家级星火计划项目，广东省科技计划项目 |
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| 摘 要： | 以香蕉为原料,采用水浸提得到粗糖液,通过SephadexG-25柱层析纯化得到香蕉低聚糖,并初步分析所得低聚糖的理化性质。结果表明:水浸提香蕉低聚糖的优化工艺条件是:料液比为(g:mL)1∶2,温度为60℃,提取时间为60min,提取2次;凝胶柱层析操作条件为:进样体积1.0mL,洗脱液流速为10s/滴,收集体积2.0mL,得到2种低聚糖;香蕉低聚糖-1为浅黄色粉末,无臭,水溶性不好,吸湿性不强;香蕉低聚糖-2为黄色粉末,无臭,水溶性良好,有一定的吸湿性;凝胶渗透色谱法测出香蕉低聚糖-2的数均分子质量为1313。
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| 关 键 词： | 香蕉 低聚糖 提取 柱层析 性质 |
Separation, Purification and Properties Researches of Banana Oligosaccharides |
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| Wang Juan,Cheng Yanfeng,Su Jinming,Yang Gongming.Separation, Purification and Properties Researches of Banana Oligosaccharides[J].Food and Fermentation Industries,2008,34(12). |
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| Authors: | Wang Juan Cheng Yanfeng Su Jinming Yang Gongming |
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| Abstract: | Banana oligosaccharides,obtained from bananas by water extraction and ethanol precipitation,were separated and purified by using the pillar SephadexG-25.The optimized processing of banana oligosaccharide extraction was :ratio of material and water(ρm:v)1∶2,60℃,extracting 60min and extracting twice.The column chromatography operation was 2.0mL/tube injection volume,distilled water as the carrier solution at a flow rate of 10s/drop,2.0mL/tube collection volume.Two kinds of oligosaccharides dehydrate powder by freeze-drying were obtained.Banana oligosaccharide-1 was light yellow powder,odorless,with lower water-solubility and moisture absorption.Banana oligosaccharide-2 is yellow powder,odorless,with higher water-solubility and moisture absorption.The molecular weight of banana oligosaccharide-2 analyzed by gel permeation chromatography was 1 313. |
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| Keywords: | banana oligosaccharide extract column chromatogram properties |
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