| 聚酰胺吸附-HPLC测定饮料和糖果中的20种水溶性色素 |
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| 引用本文： | 顾宇翔,葛宇,印杰,虞成华,陆志芸,刘孟鲲,马晓飞.聚酰胺吸附-HPLC测定饮料和糖果中的20种水溶性色素[J].食品与发酵工业,2012,38(1):161-164. |
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| 作者姓名： | 顾宇翔 葛宇 印杰 虞成华 陆志芸 刘孟鲲 马晓飞 |
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| 作者单位： | 上海市质量监督检验技术研究院／国家食品质量监督检验中心（上海）,上海,200233 |
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| 基金项目： | 国家质量监督检验检疫总局科研项目 |
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| 摘 要： | 建立了聚酰胺吸附-HPLC测定饮料和糖果中的20种水溶性色素的方法。样品用水提取,经聚酰胺粉末吸附-洗脱后,以Eclipse XDB C18柱分析(250 mm×4.6 mm,5μm),采用0.02 mol/L乙酸铵-甲醇-乙腈梯度洗脱,检测波长分别选取450、520和620 nm。该方法线性范围2.00～50.0μg/mL,重现性相对标准偏差(n=6)为0.51%～7.8%,回收率71.4%～109.2%,检出限0.03～0.3 mg/kg。
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| 关 键 词： | 聚酰胺 HPLC 饮料 糖果 色素 测定 |
Determination of 20 Water-soluble Pigments in Beverages and Candy by Polyamide |
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| Gu Yu-xiang,Ge Yu,Yin Jie,Yu Cheng-hua,Lu Zhi-yun, Liu Meng-kun,Ma Xiao-fei.Determination of 20 Water-soluble Pigments in Beverages and Candy by Polyamide[J].Food and Fermentation Industries,2012,38(1):161-164. |
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| Authors: | Gu Yu-xiang Ge Yu Yin Jie Yu Cheng-hua Lu Zhi-yun Liu Meng-kun Ma Xiao-fei |
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| Affiliation: | (Shanghai Institute of Quality Inspection and Technical Research/National Center of Supervision and Inspection on Food Products Quality(Shanghai),Shanghai 200233,China) |
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| Abstract: | Research developed a polyamide absorption-HPLC method to determine 20 kinds of water-soluble pigments in beverages and candy.Samples were extracted with water,then absorbed-desorbed by polyamide,finally analyzed by Eclipse XDB C18 column(250 mm ×4.6 mm,5 μm).The mobile phase was: 0.02 mol/L ammonium acetate – methanol-acetonitrile;wavelength was 450nm,520 nm and 620nm,respectively.A good linear relationship was obtained from 2.00 μg/mL to 50.0 μg/mL.The R.S.D.of samples reproducibility(n=6) was 0.51%~7.8%;the recovery of the method was in the range of 71.4%~109.2%;the limit of detection was 0.03~0.3 mg/kg.The method was accurate and reproducible,it was suitable for analysis of several water-soluble pigments in beverages and candy simultaneously. |
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| Keywords: | polyamide HPLC beverages candy pigments determination |
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