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微生物降解棉酚的响应面分析优化
引用本文:何涛,滕达,张海军,武书庚,齐广海,王建华.微生物降解棉酚的响应面分析优化[J].食品与发酵工业,2008,34(4):71-74.
作者姓名:何涛  滕达  张海军  武书庚  齐广海  王建华
作者单位:中国农业科学院饲料研究所,北京,100081
摘    要:利用SAS软件的Plackett-Burman设计法对微生物固态发酵脱毒棉酚的发酵参数进行筛选,得出3个影响较大的重要因素,即料水比、发酵时间、装料量,再用SAS软件二次响应面分析法进行回归分析,得到了各因素的优化水平值,经过响应面法的优化,脱毒事提高近20%。

关 键 词:棉酚  响应面法  产朊假丝酵母  优化
修稿时间:2007年10月19

Bio-detoxification Optimization for Gossypol in Cottonseed Meal by Method of Response Surface Analysis
He Tao,Teng Da,Zhang Haujun,Wu Shugeng,Qi Guanghai,Wang Jianhua.Bio-detoxification Optimization for Gossypol in Cottonseed Meal by Method of Response Surface Analysis[J].Food and Fermentation Industries,2008,34(4):71-74.
Authors:He Tao  Teng Da  Zhang Haujun  Wu Shugeng  Qi Guanghai  Wang Jianhua
Abstract:Plackett—burman and response surface analysis method were applied to optimize fermentation of biodegradation of gossypol in cottonseed meal.The results indicated the detoxification rate was mainly in- fluenced by water and material ratio,material addition,and fermentation time by Plackett—burman.In the second step,the levels of those three main factors were further optimized by using Box—Behnken design. The optimal levels of the variables were:water and material ratio 1:0.94,material addition 30.96g,fer- mentation time 62.33h.Under the optimal conditions,the detoxification rate increased by 15%.Moreover, the experiment values from optimized three batches fermentation was quite close to the expected values.
Keywords:gossypol  response surface analysis  candida utilis  optimization
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