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| 实时荧光PCR方法检测转基因豆粕的研究 | |
| 引用本文： | 卫滨,孙建霞,罗云波,姜桂传,黄亚东.实时荧光PCR方法检测转基因豆粕的研究[J].食品与发酵工业,2008,34(9). |
| 作者姓名： | 卫滨 孙建霞 罗云波 姜桂传 黄亚东 |
| 作者单位： | 1. 中国农业大学食品科学与营养工程学院,北京,100083;山东省农业管理干部学院,山东济南,250100;暨南大学生物医药开发中心,广东广州,510630 2. 中国农业大学食品科学与营养工程学院,北京,100083;山东省农业管理干部学院,山东济南,250100 3. 中国农业大学食品科学与营养工程学院,北京,100083 4. 山东省农业管理干部学院,山东济南,250100 5. 暨南大学生物医药开发中心,广东广州,510630 |
| 基金项目： | 山东省教育厅资助项目 |
| 摘 要： | 以美国、阿根廷和转基因大豆标准品为材料,利用设计的特异性引物和探针,建立了实时荧光定量PCR技术检测抗草甘膦转基因豆粕的方法,成功检测出美国和阿根廷抗草甘膦转基因豆粕的内源参照基因lectin、CaMV35S/CTP of EPSPS边界序列和NOS终止子,确定了实时荧光PCR方法检测抗草甘膦转基因豆粕的灵敏度为0.1%。 |
| 关 键 词： | 转基因豆粕 实时荧光PCR 检测 |
Study on the Detection of Genetically Modified Soybean Meal by Real Time Fluorescence PCR Method |
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| Zi Weibin,Sun Jianxia,Luo Yunbo,Jiang Guichuan,Huang Yadong.Study on the Detection of Genetically Modified Soybean Meal by Real Time Fluorescence PCR Method[J].Food and Fermentation Industries,2008,34(9). | |
| Authors: | Zi Weibin Sun Jianxia Luo Yunbo Jiang Guichuan Huang Yadong |
| Abstract: | Using the American and Argentine Soybean Meal and standard samples of genetically modified soybean as baseline materials and utilizing specially designed primers and probes,the research established the qualitative detection method of glyphosate-resistant Genetically Modified Soybean Meal by Real Time Fluorescence PCR method.The method successfully detected the endogenesis gene Lectin,CaMV35S/CTP of EPSPS boundary sequence and NOS terminator in the glyphosate-resistant Genetically Modified Soybean Meal imported from the USA and Rgentina.However,no other gene but the endogenesis gene Lectin is found in Chinese Soybean Meal.The sensitivity of the detection of glyphosate-resistant Genetically Modified Soybean Meal by Real Time Fluorescence PCR method is 0.1%. |
| Keywords: | Genetically Modified Soybean Meal Real Time Fluorescence PCR dtection |
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