| 酶联免疫吸附法(ELISA)检测婴幼儿配方食品中谷蛋白 |
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| 引用本文： | 柯燕娜,葛宇,巢强国.酶联免疫吸附法(ELISA)检测婴幼儿配方食品中谷蛋白[J].食品科技,2012(3):272-274,278. |
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| 作者姓名： | 柯燕娜 葛宇 巢强国 |
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| 作者单位： | 华东理工大学生物工程学院;上海市质量监督检验技术研究院 |
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| 摘 要： | 介绍了利用ELISA法的醇溶谷蛋白试剂盒测定婴幼儿配方食品中的谷蛋白含量。样品经处理后,用提取液提取样品中谷蛋白,提取液经离心,稀释后,用酶联免疫吸附法直接测定。检测的线性范围是5～80μg/kg,相关系数是0.9988,回收率在115.6%～120.6%之间,证明可以用酶联免疫法测定婴幼儿配方食品中的谷蛋白含量。
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| 关 键 词： | 酶联免疫吸附法 谷蛋白 致敏原 婴幼儿配方食品 |
The detection of gluten in infant formula by ELISA |
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| KE Yan-na,GE Yu,CHAO Qiang-guo.The detection of gluten in infant formula by ELISA[J].Food Science and Technology,2012(3):272-274,278. |
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| Authors: | KE Yan-na GE Yu CHAO Qiang-guo |
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| Affiliation: | 1.College of Bioengineering,East China University of Science and Technology,Shanghai 200237;2.Shanghai Institute of Quality Inspection and Technical Research,Shanghai 200233) |
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| Abstract: | This article introduces the determination of gluten in infant formula by Gliadin kit based on enzyme linked immunosorbent assay.After each sample was ground into powder using a mortar and pestle,a cocktail solution was used to extract its gluten.Then,the extract was centrifugated,diluted and determined directly by ELISA.The standard curve was linear between 5 μg/kg and 80 μg/kg with regression coefficients of 0.9988.The recovery rates were between 115.6%～120.6%.It is proved that using ELISA can determinate the content of gluten in infant formula. |
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| Keywords: | ELISA gluten allergen infant formula |
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