| 高NK活性纳豆菌的诱变选育及产酶条件的研究 |
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| 引用本文： | 黄薇,孔繁东,祖国仁,季瑛,张芳,赵晗,谭晓慧.高NK活性纳豆菌的诱变选育及产酶条件的研究[J].食品科技,2007,32(4):17-21. |
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| 作者姓名： | 黄薇 孔繁东 祖国仁 季瑛 张芳 赵晗 谭晓慧 |
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| 作者单位： | 大连轻工业学院生物与食品工程学院,大连,116034 |
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| 基金项目： | 辽宁省教育厅A类科技基金 |
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| 摘 要： | 采用物理化学诱变方法,对纳豆菌进行复合诱变,以期筛选出纳豆激酶(NK)活力高的优良菌株。以实验室分离、筛选的纳豆菌NK-8作为出发菌,通过紫外线和氯化锂对其进行复合诱变,经过菌种的初筛和复筛,得到了酶活较出发菌提高2.2倍的菌株JN-14,并对初始菌株与诱变菌株进行了一系列的比较。实验进一步研究了该菌株产纳豆激酶的发酵过程,比较了不同的营养源与生长条件对菌株产酶的影响,优化、确定了菌株产纳豆激酶的产酶条件。
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| 关 键 词： | 纳豆菌 纳豆激酶 诱变育种 发酵条件 |
| 文章编号： | 1005-9989(2007)04-0017-05 |
| 修稿时间： | 2006年10月1日 |
High NK activity of Bacillus natto by mutation breeding and study on culture conditions of nattokinase production |
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| HUANG Wei,KONG Fan-dong,ZU Guo-ren,JI Ying,ZHANG Fang,ZHAO Han,TAN Xiao-hui.High NK activity of Bacillus natto by mutation breeding and study on culture conditions of nattokinase production[J].Food Science and Technology,2007,32(4):17-21. |
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| Authors: | HUANG Wei KONG Fan-dong ZU Guo-ren JI Ying ZHANG Fang ZHAO Han TAN Xiao-hui |
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| Abstract: | The Bacillus natto was mutated by physical or chemical methods to improve nattokinase(NK) activity. An strain of B.subtilis NK-8, isolated from our laboratory, was mutated by UV rays and Lithium Chloride. A mutant JN-14 producing 2.2 times more nattokinase activity than its parent strain was obtained after preliminary screening and ambo-sieving. The influences of different nutrient sources and growth medium on JN-14 were studied, and the fermentation condition of producing nattokinase was optimized and determined. |
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| Keywords: | Bacillus natto nattokinase mutation breeding fermentation condition |
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