戴氏虫草水提多糖和碱提多糖的活性炭脱色工艺优化

目的:以贵州特色药用真菌戴氏虫草为实验材料，探究其水提多糖和碱提多糖的活性炭脱色工艺。方法:选取活性炭用量、脱色时间、脱色温度和脱色pH进行单因素实验，在此基础上通过四因素三水平正交试验分别对两种多糖活性炭脱色的最佳工艺进行优选。结果:戴氏虫草水提多糖和碱提多糖的最佳脱色条件分别为活性炭用量1.5和2 g/100 mL，脱色时间10和30 min，脱色温度50和70 ℃，脱色pH为4和8。各自最优条件下，水提多糖的脱色率和多糖保留率分别为92.12%±0.45%和73.46%±0.33%，碱提多糖的脱色率和多糖保留率分别为75.67%±0.66%和56.72%±0.47%。结论:活性炭吸附法对戴氏虫草水提多糖和碱提多糖具有明显的脱色效果，且多糖保留率高。该脱色工艺简单高效，成本低廉，适合工业化应用。

Optimization of Activated Carbon Decolorization Process of Water- and Alkali-Extracted Polysaccharides from Cordyceps taii

Objective: Cordyceps taii is a kind of characteristic medical fungus resource in Guizhou. In this study, the decolorization process of activated carbon for water- and alkali-extracted polysaccharides from C. taii was explored. Methods: Based on the single factor experiment of active carbon dosage, decolorization time, decolorization temperature and decolorization pH, the optimum decolorization processes of two kinds of polysaccharide with active carbon were optimized by four factors on three levels orthogonal experiment. Results: Optimal decolorization technology of water- and alkali-extracted polysaccharides were as follows: Active carbon dose were 1.5 and 2 g/100 mL, decolorization time were 10 and 30 min, decolorization temperature were at 50 and 70 °C, decolorization pH were at 4 and 8, respectively. Under the optimal conditions, the decolorization rate and the retention rate of water-extracted polysaccharides were 92.12%±0.45% and 73.46%±0.33%, and the decolorization rate and the retention rate of alkali-extracted polysaccharides were 75.67%±0.66% and 56.72%±0.47%, respectively. Conclusion: Active carbon adsorption had significant decolorization effect on water- and alkali-extracted polysaccharides from C. taii, and the retention rates of polysaccharides were both high. The decolorization process was simple, efficient, low cost and suitable for industrial application.