6′′′-阿魏酰斯皮诺素对Aβ1-42诱导损伤的SH-SY5Y细胞保护作用研究

目的:探讨酸枣仁黄酮类成分6′′′-阿魏酰斯皮诺素预处理对Aβ_1-42诱导损伤SH-SY5Y细胞的保护作用。方法:利用6′′′-阿魏酰斯皮诺素（1、5、10、20和40 μmol/L）预处理SH-SY5Y细胞2 h，随后加入5 μmol/L的Aβ_1-42共同孵育24 h，利用CCK-8法检测细胞活力；Calcein-AM/PI双染法观察细胞形态；试剂盒检测细胞内活性氧、丙二醛、线粒体膜电位水平及谷胱甘肽过氧化物酶活力；蛋白免疫印迹法检测细胞凋亡相关蛋白:Bcl-2关联死亡启动子重组蛋白、B细胞淋巴瘤/白血病-2蛋白的表达。结果:6′′′-阿魏酰斯皮诺素可以抑制Aβ_1-42诱导的细胞凋亡，提高细胞活力(P<0.001)；改善Aβ_1-42诱导损伤细胞的氧化应激情况，降低细胞内活性氧(P<0.001)、丙二醛水平(P<0.01)，提高谷胱甘肽过氧化物酶活力(P<0.001)；改善Aβ_1-42诱导损伤的细胞线粒体功能，上调细胞线粒体膜电位水平(P<0.001)；增加抗凋亡蛋白B细胞淋巴瘤/白血病-2蛋白的表达(P<0.01)，并降低促凋亡蛋白Bcl-2关联死亡启动子重组蛋白的表达(P<0.01)。结论:6′′′-阿魏酰斯皮诺素对Aβ_1-42诱导损伤的SH-SY5Y细胞具有保护作用，其机制与6′′′-阿魏酰斯皮诺素抗氧化活性及调节凋亡相关蛋白表达有关。

Protective Effect of 6′′′-Feruloylspinosin on Aβ1-42-Induced SH-SY5Y Cells Injury

Objective: To investigate the protective effects of 6′′′-feruloylspinosinon Aβ_1-42-induced SH-SY5Y cells injury. Methods: SH-SY5Y cells were pretreated with 6′′′-feruloylspinosin (1、5、10、20 and 40 μmol/L) for 2 h and then co-treated with 5 μmol/L of Aβ_1-42 for 24 h. Cell viability was measured by CCK-8 assay; cell morphology was observed by Calcein-AM/PI assay; intracellular reactive oxygen species, malonic dialdehyde level, Glutathione peroxidaseactivity and mitochondrial membrane potential level were detected by the kit; expression of apoptosis-related proteins: Bcl-xL/Bcl-2 associated death promoter, B-cell lymphoma/leukemia-2 were detected by Western Blotting. Results: 6′′′-feruloylspinosin could inhibit Aβ_1-42-induced apoptosis and improve cell viability (P<0.001); reduce intracellular reactive oxygen species (P<0.001) and malondialdehyde (P<0.01) levels; increase glutathione peroxidase activity (P<0.001); upregulate cell mitochondrial membrane potential level (P<0.001); regulate apoptosis-related protein expression (P<0.01). Conclusion: The results validated that 6′′′-feruloylspinosin had a protective effect on SH-SY5Y cells with Aβ_1-42-induced damage, and the mechanism was that 6′′′-feruloylspinosin could alleviate oxidative damage and regulate the expression of apoptosis related proteins.