屎肠球菌B21对高脂饮食Wistar大鼠体重、空腹血糖和粪便菌群的影响

本研究分析了摄食屎肠球菌(Enterococcus faecium)B21菌株对高脂饮食Wistar大鼠体重和空腹血糖的影响;并运用高通量测序技术分析了大鼠肠道菌群多样性的变化。36只Wistar大鼠随机分为正常对照组(NCD)、高脂饮食组(HFD)和E. faecium B21干预组(HFD+B21)。NCD组大鼠喂食普通饲料,HFD组和HFD+B21组大鼠喂食高脂饲料。自第1周起,给予HFD+B21组每只大鼠每天灌胃0.2 mL E. faecium B21(108 CFU/mL)至第7周。收集第7周大鼠粪便,通过高通量测序分析了3组大鼠肠道菌群的结构和组成。结果表明:在第7周时,HFD+B21组(366.51 g)大鼠体重显著高于HFD组(339.43 g,p<0.05)和NCD组(306.84 g,p<0.05),HFD+B21组大鼠(7.63 mmol/L)空腹血糖显著低于HFD组(8.75 mmol/L,p<0.05),且与NCD组无显著性差异(7.08 mmol/L,p>0.05)。HFD+B21组大鼠肠道菌群丰度(ACE值398.12,Chao1值398.49)和多样性最低(Shannon指数3.83),NCD组菌群丰度(ACE值700.57,Chao1值698.07)和多样性(Shannon指数5.06)最高;E. faecium B21的摄入降低了菌群中致病菌属假单胞菌属(Pseudomonas)和脱硫弧菌属(Desulfovibrio)的比率,同时促进了有益菌属布劳特氏菌属(Blautia)的增长。结论:E. faecium B21的摄入能促进高脂饮食Wistar大鼠的体重,降低其空腹血糖且对肠道菌群失衡具有一定的调节作用。

Effects of Enterococcus faecium B21 on Body Weight,Fasting Blood Glucose Levels,and Fecal Bacterial Communities of Wistar Rats Fed by High Fat Diet

The aim of this study was to investigate the effects of Enterococcus faecium B21 on body weight, fasting blood glucose levels, and fecal bacterial communities of high fat diet (HFD) -treated Wistar rats. Thirty-six Wistar rats were randomly divided into three groups, including the normal chow-diet (NCD) group, HFD group, HFD and E. faecium B21 (HFD+B21) group. Each rat in the HFD+B21 group was fed with 0.2 mL E. faecium B21 (108CFU/mL) each day. After the intragastric administration for 7 weeks, the feces of the rats were collected, and their fecal bacterial communities were detected by high-throughput sequencing of bacterial 16S rRNA gene. The results showed that body weight of the rats in the HFD+B21 group (366.51 g) were increased significantly compared with those in the groups HFD (339.43 g, p<0.05) and NCD (306.84 g, p<0.05), and the fasting blood glucose levels of rats in the HFD+B21 group (7.63 mmol/L) were reduced significantly compared with those in the HFD group (8.75 mmol/L, p<0.05) and there was no significant difference compared with those in the NCD group (7.08 mmol/L, p>0.05). The HFD+B21 group had the lowest bacterial abundance (ACE-index 398.12, Chao1-index 398.49) and diversity (Shannon index 3.83), while the NCD group had the highest abundance (ACE-index 700.57, Chao1-index 698.07) and diversity (Shannon index 5.06). The results also displayed that the intake of E. faecium B21 increased the ratios of probiotics Blautiain the bacterial population, and inhibited the growth of pathogenic bacteria Pseudomonas and Desulfovibrio. These results suggested that E. faecium B21 had a hypoglycemic effect and regulated the imbalance of intestinal bacterial population in Wistar rats caused by the intake of HFD.