普洱茶挥发性组分的抗癌、抗炎功能特性

为探究普洱茶挥发性组分中抗癌、抗炎活性组分,本研究以云南大叶种加工的普洱茶为材料,采用同时蒸馏萃取法(SDE)、化学法和薄层层析法(TLC)提取、分离茶叶挥发性组分。通过气相色谱-质谱法分析鉴定挥发性组分的组成和含量;采用小鼠肝癌细胞Hepa1c1c7诱导醌还原酶(QR)活性倍增的CD值和LPS刺激小鼠巨噬细胞RAW264.7对NO抑制率的IC₅₀值评价、筛选抗癌、抗炎活性组分;选取抗癌、抗炎活性组分中的7种单体物质,通过抗癌、抗炎细胞模型确定其功能特性。结果表明,普洱茶挥发性组分中,诱导QR活性较强的有初提组分、中性组分、TLC分离的F6和F6-2组分,其CD值分别为28.07、45.31、12.8、15.12 μg/mL;抑制NO活性较强的为初提组分和中性组分,其IC₅₀值分别为51.68、73.32 μg/mL;β-紫罗酮、1,2,3-三甲氧基苯、1,2,4-三甲氧基苯和1,2-二甲氧基苯在12.5~800.0 μg/mL浓度范围内同时具有抗癌、抗炎活性;植酮、α-松油醇和叶绿醇在200~800 μg/mL浓度范围内具有抗癌活性。上述研究结果说明,普洱茶挥发性组分具有抗癌、抗炎功能活性;β-紫罗酮、甲氧基苯类物质等是其功能活性组分。

Anti-cancer and Anti-inflammatory Activities of Volatile Components from Pu-erh Tea

To investigate the anti-cancer and anti-inflammatory activities of volatile components in Pu-erh tea from Yunnan Daye variety, crude aroma was extracted by simultaneous distillation-extraction (SDE), and further isolated using chemical method and thin layer chromatography method (TLC), and gas chromatography-mass spectrometry (GC-MS) was applied to analyze aroma components. The CD values of the double-induced quinone reductase activity induced by murine hepatoma cell (Hepa1c1c7) and the IC₅₀ values of the inhibitory rate of nitric oxide (NO) by lipopolysaccharide (LPS) -stimulated mouse macrophage (RAW264.7) cell were used to evaluate and screen the anti-cancer and anti-inflammatory components. Results showed that, in the volatile components of Pu-erh tea, the crude aroma, neutral component, F6, and F6-2 exhibited strong QR inducing activities, and their CD values were 28.07, 45.31, 12.8 and 15.12 μg/mL, respectively. Furthermore, the crude aroma and neutral component possessed strong NO inhibitory activities, and the IC₅₀ values were 51.68 and 73.32 μg/mL, respectively. Additionally, among the main monomers, β-ionone, 1, 2, 3-trimethoxybenzene, 1, 2, 4-trimethoxybenzene, and 1, 2-dimethoxybenzene possessed anti-cancer activities and anti-inflammatory activities in the concentration range of 12.5~800.0 μg/mL, meanwhile. However, 2-pentadecanone, phytol, and α-terpineol displayed anti-cancer activities in the concentration range of 200~800 μg/mL. The above results showed that the volatile components of Pu-erh tea had anticancer and anti-inflammatory activities, and β-ionone and methoxybenzene components were their active components.