枣种质资源果实cAMP和cGMP含量分析

了解不同枣种质资源环磷酸腺苷（cyclic adenosine monophosphate，cAMP）和环磷酸鸟苷（cyclic guanosine monophosphate，cGMP）含量特征，筛选含量较高的种质，为资源开发利用提供参考。以204份枣种质资源为试材，利用超高效液相色谱法（Ultra-high performance liquid chromatography，UPLC）测定各种质脆熟期果实的cAMP和cGMP含量，并进行变异分析、相关性分析及聚类分析，进而筛选cAMP和cGMP含量较高的种质。结果表明，同一种质的cAMP含量高于cGMP，204份种质cAMP和cGMP含量的平均值分别为58.01 μg·g-1·FW和22.82 μg·g-1·FW，变异系数分别为53.77%和58.81%，二者含量均服从正态分布；cAMP含量和cGMP含量间呈极显著正相关；聚类分析将204份枣种质资源在遗传距离为4.5处划分为3类，分别定义为高含量类群、中含量类群和低含量类群，筛选出溆浦香枣、临泽大枣、临猗笨枣、根德大枣、延川跌牙枣等28份cAMP和cGMP含量较高的枣种质。初步建立了枣种质资源cAMP和cGMP含量的分级指标，并筛选出含量较高的种质资源，为cAMP和cGMP功能食品开发提供基础原料。

Analysis of cAMP and cGMP Contents in Fruits of Chinese Jujube Germplasm Resources

This study aims to understand the characteristics of cyclic adenosine monophosphate(cAMP)and cyclic guanosine monophosphate(cGMP)contents in different jujube germplasm resources and select the higher content germplasm,thus to provide reference for further development and utilization resources.The contents of cAMP and cGMP in crisp mature fruits of 204 jujube germplasm resources were determined by using ultra high performance liquid chromatography(UPLC),and variation,correlation and cluster analysis were performed.The results showed that cAMP content was higher than cGMP for the same resource.The mean contents of cAMP and cGMP in crisp mature fruits of 204 resources were 58.01μg·g^-1·FW and 22.82μg·g^-1·FW,the variation coefficient were 53.77%and 58.81%,respectively.Both of them were normally distributed.The cAMP content significanty positive correlation with the cGMP content.Cluster analysis in 4.5 genetic distance can divide 204 resources into 3 groups,which were defined as high content group,middle content group and low content group,respectively.28 jujube resources with high contents of cAMP and cGMP,including‘Xupuxiangzao’,‘Linzedazao’,‘linyibenzao’,‘Gendedazao’,‘Yanchuandieyazao’and so on,were screened out.In this study,we established the classification index for jujube cAMP and cGMP contents and selected the specific germplasms with higher contents,which can provide a theoretical basis for the selection of foundation materials for the processing of cAMP and cGMP functional food.