人参、甘草、五味子复合醋提物对老年小鼠急性炎性肝损伤保护作用的研究

目的:本研究旨在通过脂多糖（Lipopolysaccharide，LPS）诱导肝损伤小鼠考察复合醋提物（MVE）对老年小鼠炎性肝损伤的保护效果。方法:将小鼠随机分为正常对照组、MVE组、LPS组、LPS+MVE组。通过苏木精-伊红染色评估肝组织病理情况和免疫组织化学染色判断髓过氧化物酶的表达变化。同时，检测肝组织中白介素-1β、白介素-17、CXC趋化因子配体1、CXC趋化因子配体17等生化因子水平。免疫印迹法测定肝脏中Toll样受体4（Toll-like receptor 4，TLR4）和p-NF-κB p65蛋白表达量变化。结果:复合醋提物能减少中性粒细胞及炎症细胞浸润，极显著降低LPS模型小鼠肝脏谷丙转氨酶（ALT）、谷草转氨酶（AST）、活性氧（ROS）水平（P<0.01），并降低促炎因子和中性粒细胞趋化因子水平（P<0.05），降低炎症通路TLR4和p-NF-κB p65的蛋白表达水平（P<0.001）。结论:MVE能改善LPS诱导急性炎症小鼠的肝损伤，此外，MVE还能通过平衡促炎和抗炎因子水平变化、抑制TLR4-NF-κB信号通路，最终达到发挥护肝作用效果。

Protective Effect of Medicine Vinegar Extract of Panax ginseng,Glycyrrhiza and Schisandra chinensis on Acute Liver Injury in Mice

Objective:Investigating the protective effect of MVE was the purpose of this study,which performed on LPSinduced liver injury in aged mice.Methods:The mice were randomized into the groups of normal control,MVE,LPS and LPS+MVE.The pathological changes of liver tissue and the expression of myeloperoxidase were observed by hematoxylineosin staining and immunohistochemical staining.The levels of IL-1β,IL-17,CXCL1,CXCL17 and other factors in liver were measured.Western blotting was used to detect the expressions of TLR4 and NF-κB p65 in liver tissue samples.Results:The MVE could effectively decline the infiltration of neutrophils and inflammatory cells,significantly decrease the levels of ALT,AST,ROS(P<0.01),pro-inflammatory cytokines and neutrophil chemokines(P<0.05)in the liver of LPS model mice,and decrease protein expressions of TLR4 and NF-κB p65 in inflammatory pathway(P<0.001).Conclusion:The MVE could improve the liver injury of mice with acute inflammation induced by LPS,and exert its hepatoprotective mechanism by regulating the balance of pro-inflammatory and anti-inflammatory factors and inhibiting TLR4-NF-κB signal pathway.