响应面法优化大孔树脂纯化黄芪毛蕊异黄酮工艺

为优化大孔树脂纯化黄芪毛蕊异黄酮提取物的最佳工艺条件，比较七种不同类型大孔树脂（H103、D101、AB-8、DM130、HPD-400、DM301、HPD-600）的静态吸附-洗脱性能，筛选合适树脂型号后，采用单因素与响应面试验确定最佳纯化工艺条件。结果表明，HPD-400树脂对毛蕊异黄酮的吸附纯化效果最佳。随着温度的升高，树脂吸附量下降，吸附过程符合二级动力学模型特征。大孔树脂纯化黄芪毛蕊异黄酮的最佳工艺为:质量浓度为2.97 mg/mL，pH4.9的毛蕊异黄酮提取液60 mL以1 mL/min流速上样至HPD-400树脂后，经140 mL体积分数为79.8%乙醇溶液，以1 mL/min流速洗脱，产物中毛蕊异黄酮含量由2.17%提高至10.36%，约为纯化前4.8倍。因此，该工艺条件适于黄芪毛蕊异黄酮纯化。

Purification of Calycosin from Astragalusme mbranceus with Macroporous Resins by Response Surface Analysis

To optimize the technical condition for purification of calycosin of Astragalus membranceus with macroporous resin, the static adsorption and desorption performance of macroporous resin of seventy types （H103, D101, AB-8, DM130, HPD-400, DM301, HPD-600） were compared for select the best type resin. The optimum process condition was obtained by single factor experiments and response surface experiment. The results showed that HPD-400 macroporous resin was the best macroporous resin for purification of calycosin of Astragalus membranceus, and the adsorption quantity of the resin was gradual decreasing with temperature increasing, and the adsorption process fitted characteristic of second-order kinetics model. The optimum process condition were as follows: The initial concentration of sample solution was 2.97 mg/mL, the volume was 60 mL with loading speed of 1.0 mL/min, the pH of sample solution was 4.9, and the volume fraction of ethanol was 79.8%, the eluent volume was 140 mL with flow rate of 1.0 mL/min. The contents of calycosin in product increased from 2.17% to 10.36%, which was about 4.8 times of that before purification. Therefore, the HPD-400 macroporous resin had good performance for separation and purification of calycosin from extracts, the purification process obtained in this study was suitable for the purification of Astragalus membranceus calycosin.