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椰子吸器多糖的结构和体外生物学活性分析
引用本文:张玉锋,陈颖娴,温远芬,唐敏敏,宋菲,朱婷婷,张有林.椰子吸器多糖的结构和体外生物学活性分析[J].食品工业科技,2021,42(17):78-84.
作者姓名:张玉锋  陈颖娴  温远芬  唐敏敏  宋菲  朱婷婷  张有林
作者单位:1.中国热带农业科学院椰子研究所,国家重要热带作物工程技术中心(椰子研发部),海南省椰子深加工工程技术研究中心,海南文昌 5713392.陕西师范大学食品工程与营养科学学院,陕西西安 7101193.海南热带海洋学院食品科学与工程学院,海南三亚 572022
基金项目:海南省重点研发计划(ZDYF2019108);中国热带农业科学院基本科研业务费专项资金(1630152021001)
摘    要:采用水提醇沉法制备了椰子吸器多糖,并初步分析了其单糖组成、分子量大小与分布、红外光谱等结构特征和抗氧化、降糖酶抑制等体外生物学活性。结果表明:吸器多糖是一种酸性杂多糖,中性糖和糖醛酸含量分别为42.33%和21.34%,可能含有吡喃环结构,主要由岩藻糖、鼠李糖、阿拉伯糖、半乳糖、葡萄糖、木糖和半乳糖醛酸7种单糖组成,摩尔比为1.00:4.40:21.27:21.07:51.76:7.06:4.33。分子量分析表明吸器多糖由3个组分构成,其中峰3为主成分,分子质量为1.53×104 g/moL。吸器多糖在6 mg/mL对DPPH和羟自由基的清除率分别为82.49%和85.83%,100 mg/mL时对α-葡萄糖苷酶抑制率达69.91%。研究结果可为椰子吸器在功能性食品或医药等领域的加工利用提供数据支撑。

关 键 词:椰子吸器多糖    清除自由基    单糖组成    结构分析    α-葡萄糖苷酶抑制活性
收稿时间:2021-01-25

Structure and Biological Activities in Vitro Analysis of Polysaccharide from Coconut (Cocos nucifera L.) Haustorium
ZHANG Yufeng,CHEN Yingxian,WEN Yuanfen,TANG Minmin,SONG Fei,ZHU Tingting,ZHANG Youlin.Structure and Biological Activities in Vitro Analysis of Polysaccharide from Coconut (Cocos nucifera L.) Haustorium[J].Science and Technology of Food Industry,2021,42(17):78-84.
Authors:ZHANG Yufeng  CHEN Yingxian  WEN Yuanfen  TANG Minmin  SONG Fei  ZHU Tingting  ZHANG Youlin
Affiliation:1.Coconut Research Institute of Chinese Academy of Tropical Agricultural Sciences, National Center of Important Tropical Grops Engineering and Technology Research (Coconut Center), Hainan Engineering Center of Coconut Further Processing, Wenchang 571339, China2.College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710119, China3.College of Food Science and Engineering, Hainan Tropical Ocean University, Sanya 572022, China
Abstract:Coconut haustorium polysaccharide (CHP) was prepared by the method of water extraction and alcohol precipitation, its structural characteristics consist of monosaccharide composition, molecular weight size and distribution and infrared spectrum and biological activities in vitro such as antioxidant and hypoglycemic enzyme inhibiting activities were also analyzed. Results showed that CHP was an acidic heteropolysaccharide with the content of neutral sugar and uronic acid of 42.33% and 21.34%, which might contain pyran ring structure. Monosaccharide composition analysis indicated that CHP was mainly composed of fucose, rhamnose, arabinose, galactose, glucose, xylose and galacturonic acid and the molar ratio was 1.00:4.40:21.27:21.07:51.76:7.06:4.33. Molecular weight analysis showed that the CHP was composed of three components, of which peak 3 was the main component with the molecular weight of 1.53×104 g/moL. Besides, DPPH and hydroxyl radical scavenging rate of CHP at 6 mg/mL were 82.49% and 85.83%, respectively. And its inhibition rate on α-glucosidase was 69.91% at 100 mg/mL. These research results could provide data support for the processing and utilization of coconut haustorium in functional food or medicine.
Keywords:
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