| 几种单糖、寡糖TLC条件的确立及草石蚕寡糖中水苏糖含量测定 |
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| 引用本文： | 沈德艳,郁建平,张训海,王旋,时维静,蔡立,杨文锟.几种单糖、寡糖TLC条件的确立及草石蚕寡糖中水苏糖含量测定[J].食品工业科技,2009(9). |
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| 作者姓名： | 沈德艳 郁建平 张训海 王旋 时维静 蔡立 杨文锟 |
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| 摘 要： | 目的:建立薄层色谱(TLC)分析几种单糖、寡糖的有效方法,并测定草石蚕寡糖中水苏糖含量.方法:以果糖、蔗糖、棉子糖和水苏糖为研究对象,从斑点分离度、斑点集中与否、有无拖尾现象及展开时间几方面探讨其在不同体系下的薄层色谱行为;通过薄层色谱扫描(TLCS)测定草石蚕寡糖中水苏糖含量.结果:确立适合的展开系统为乙酸乙酯/甲醇/水/冰乙酸(11.5:3.2:2:3.2),合适的显色剂为茴香醛-浓硫酸显色剂;草石蚕寡糖中水苏糖含量为69.3%.
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| 关 键 词： | 草石蚕 单糖 寡糖 水苏糖 TIE TLCS |
Establishment of TLC conditions for several monosaccharide and oligosaccharide and determination the content of stachyose in oligosaccharide from Stachys seiboibi Miq |
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| SHEN De-yan,YU Jian-ping,ZHANG Xun-hai,WANG Xuan,SHI Wei-jing,CAI Li,YANG Wen-kun.Establishment of TLC conditions for several monosaccharide and oligosaccharide and determination the content of stachyose in oligosaccharide from Stachys seiboibi Miq[J].Science and Technology of Food Industry,2009(9). |
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| Authors: | SHEN De-yan YU Jian-ping ZHANG Xun-hai WANG Xuan SHI Wei-jing CAI Li YANG Wen-kun |
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