无蔗糖山楂果丹皮对小鼠便秘的预防作用

本研究采用低聚木糖和木糖醇按照25:75、50:50和75:25的比例替代传统果丹皮中的蔗糖制备无蔗糖山楂果丹皮,并评价其感官特性和对盐酸地芬诺酯诱导的便秘昆明小鼠的预防效果。结果表明,当低聚木糖占总糖的25%或50%时,该果丹皮在质构、色差及感官评价方面与传统蔗糖果丹皮相比无显著差异(P>0.05)。低聚木糖/木糖醇(50:50)复合无蔗糖山楂果丹皮中、高剂量组相比低聚木糖果丹皮组及木糖醇果丹皮组,预防便秘效果更加显著(P<0.05)。与模型组相比,低(3 g/kg·bw/d)、中(6 g/kg·bw/d)和高剂量(9 g/kg·bw/d)低聚木糖/木糖醇(50:50)复合果丹皮可显著提高便秘小鼠的小肠推进率(76.0%、108.9%和83.8%)和6 h黑便湿重(65.4%、82.7%和93.3%);胃动素(MTL)含量分别增加24.0%、26.6%和25.1%;乳酸、乙酸和丙酸三种有机酸总含量分别增加68.7%、72.8%和121.6%。总之,低聚木糖/木糖醇(50:50)复合无蔗糖山楂果丹皮低、中、高剂量均能预防小鼠便秘,且中、高剂量组的效果更好。

Preventive Effects of Sugar-Free Hawthorn Rolls on Constipation in Mice

In this study,xylooligosaccharide(XOS)and xylitol(Xyl)with the ratio of 25:75,50:50 and 75:25 were used to prepare sugar-free hawthorn rolls by replacing sucrose. The sensory characteristic of sugar-free hawthorn rolls and its preventive effects on diphenoxylate-induced constipation in Kunming mice were estimated. The texture,chromatic aberration and sensory evaluation of sugar-free hawthorn rolls with XOS accounting for 25% or 50% of the total sugar were not significantly different with those of the traditional hawthorn rolls containing sucrose(P>0.05). Compared with XOS and Xyl,the middle-dose and high-dose groups of hawthorn rolls substituted with 50% XOS and 50% Xyl had significant higher preventive effects on constipation in mice(P<0.05). Compared with model group,small intestinal propulsion rate of constipated mice fed with low(3 g/kg·bw/d),middle(6 g/kg·bw/d)and high-dose(9 g/kg·bw/d)of XOS/Xyl(50:50)substitution was increased by 76.0%,108.9% and 83.8%,respectively,and the wet weights of defecation within 6 hours were increased by 65.4%,82.7% and 93.3%,respectively. Accordingly,MTL level was increased by 24.0%,26.6% and 25.1%,respectively,and the total contents of lactic acid,acetic acid,and propionic acid were increased by 68.7%,72.8% and 121.6%,respectively. Taken together,the low,middle and high-dose administration of XOS/Xyl(50:50)substituted hawthorn rolls have preventive effect on constipation in mice,especially for the middle and high-dose groups.