| 紫外分光光度法测定烟草中的游离烟碱 |
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| 引用本文： | 殷全玉,杨铁钊,郭宝银,黄进慧.紫外分光光度法测定烟草中的游离烟碱[J].中国烟草科学,2008,29(6). |
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| 作者姓名： | 殷全玉 杨铁钊 郭宝银 黄进慧 |
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| 作者单位： | 1. 河南农业大学农学院,河南,郑州,450002
2. 重庆酉阳烟草公司,重庆,酉阳,409800 |
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| 摘 要： | 研究了用三氯甲烷萃取、紫外分光光度计检测烟草叶片游离烟碱含量的方法,探讨了三氯甲烷提取烟草游离烟碱的用量和萃取时间。结果表明50mL三氯甲烷振荡萃取30min可以将1g烟草样品游离烟碱萃取完全。本方法测烟叶游离烟碱回收率97.7%,重复性良好。
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| 关 键 词： | 烟叶 游离烟碱 紫外分光光度法 |
Determination of Free Nicotine in Tobacco Leaves by UV Spectrophotometry |
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| YIN Quanyu,YANG Tiezhao,GUO Baoyin,HUANG Jinhui.Determination of Free Nicotine in Tobacco Leaves by UV Spectrophotometry[J].Chinese Tobacco Science,2008,29(6). |
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| Authors: | YIN Quanyu YANG Tiezhao GUO Baoyin HUANG Jinhui |
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| Affiliation: | YIN Quanyu1,YANG Tiezhao1,GUO Baoyin2,HUANG Jinhui1 |
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| Abstract: | This paper deals with the determination of free nicotine in tobacco leaves by modified chloroform extraction and UV spectrophotometer,and the optimum extraction time and dose of chloroform.The results indicated that the free nicotine in 1g tobacco sample could be entirely extracted by 50 mL CHCl3 in 30 min.By use of UV spectrophotometry,the recovery of free nicotine in tobacco leaves reached 88.4% with high repeatability. |
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| Keywords: | tobacco leaves free nicotine UV spectrophotometry |
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