不同品种雪茄烟叶晾制后代谢组的差异分析

不同品种雪茄烟叶晾制后叶色存在显著差异。本研究以晾制后的雪茄烟品种楚雪10号（CX-010）和楚雪14号（CX-014）烟叶为材料，运用液相色谱-串联质谱（LC-MS/MS）联用技术开展非靶向代谢组学分析，探索两品种烟叶在晾制后代谢物的相对含量差异及其与叶色的关系。结果表明，两个品种晾制后的烟叶代谢物相对含量存在显著差异。与CX-014相比，CX-010烟叶中游离氨基酸与生物碱相对含量总体较低，脂质相对含量总体较高，相差最大的32种差异代谢物包含2种氨基酸、10种酚酸、3种酰胺等物质。结合KEGG代谢通路分析，发现精氨酸和脯氨酸代谢通路中脯氨酸生物合成途径在两品种间差异显著。差异代谢物的生理功能分析显示，两品种烟叶晾制后的叶色差异主要与脯氨酸、水杨酸、黄酮类和生物碱的含量差异有关。

Differential Analysis of Metabolomics of Cigar Tobacco Leaves of Different Varieties after Air-curing

there were significant differences in leaf color between different cigar tobacco varieties after air-curing. In this study, the non-targeted metabolomic analysis was performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to investigate the relative abundance of metabolites and their relationship with the differences in leaf color after air-curing of cigar tobacco varieties Chuxue No. 10 (CX-010) and Chuxue No. 14 (CX-014). The results showed that there were significant metabolite abundance differences between tobacco leaves of the two varieties after air-curing. Compared with CX-014, the relative abundance of free amino acids and alkaloids in CX-010 tobacco leaves was generally lower, and the overall lipid content was higher. 32 different metabolites with the largest difference in difference multiplicity contained 2 amino acids, 10 phenolic acids, 3 amides and other substances. Combined with the analysis of the KEGG metabolic pathway, it was found that the proline biosynthesis pathway in the arginine and proline metabolic pathways differed significantly between the two varieties. The physiological function analysis of the differential metabolites showed that the differences in leaf color between the two varieties after air-curing might be mainly related to the differences in the contents of proline, salicylic acid, and flavonoids and alkaloids.