微波辅助分步酶解菜籽粕制备菜籽多肽的研究

以脱脂菜籽粕为原料,利用微波辅助技术,对碱性蛋白酶和风味蛋白酶分步酶解菜籽粕蛋白的工艺进行了研究。结果表明,在最适微波条件下(碱性蛋白酶的微波温度46℃,风味蛋白酶50℃,微波功率均为500 W),碱性蛋白酶加酶量9 000 U/g,酶解3 min,风味蛋白酶加酶量37.5 LAPU/g,酶解13 min,得到的酶解产物的水解度为50.94%,氮收率为96%,多肽得率34.45%,氮溶指数84.22%,三氯乙酸氮溶指数77.90%。凝胶柱层析法分析表明,酶解产物为大量相对分子质量在1 000 Da的短肽。

Microwave-assisted step enzymatic hydrolysis of rapeseedmeal to prepare rapeseed peptides

The step enzymatic hydrolysis process assisted with microwave of rapeseed meal was studied by Alcalase and Flavourzyme to prepare rapeseed peptides.The results showed that under the optimum conditions,the optimum conditions were determined that the microwave power for the two protases was 500 W,and the microwave temperature was 46 ℃ for Alcalase and 50 ℃ for Flavourzyme;the enzyme dosage was 9 000 U/g for Alcalase and 37.5 LAPU/g for Flavourzyme,and the hydrolysis time was just 3 min for Alcalase and 13 min for Flavourzyme.Under the optimum conditions,the analysis of gel permeation chromatography proved the relative molecular weight of main hydrolysate was 1 000 Da.