Relative quantification of <Emphasis Type="Italic">Vitis vinifera</Emphasis> L. varieties in musts by microsatellite DNA analysis |
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Authors: | Miguel Angelo Faria Eugénia Nunes Maria Beatriz P P Oliveira |
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Affiliation: | 1.REQUIMTE, Servi?o de Bromatologia, Faculty of Pharmacy,University of Porto,Porto,Portugal;2.CIBIO, Laboratory of Vegetal Genetics, Faculty of Sciences,Universitiy of Porto,Vila do Conde,Portugal |
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Abstract: | Varietal wines are defined as made primarily from a single variety of grape, which is identified on the label. However the
inclusion of other varieties in their production is permitted under defined percentages. The fact that different grape varieties/varietal
wines present far different economical values can attract fraudulent practices. Thus the development of methods, which allow
the quantification of those varieties, is of crucial importance. The first approach to the method for the relative quantification
of must containing two varieties based on microsatellite DNA analysis is presented. Quantification was achieved by densitometry
measurements of the amplification products of the pooled varieties, obtained with loci VVMD6 and VVMD7, after their separation and staining on polyacrilamide gels. We have found that a good correlation between
the proportion of each variety in must and the signal intensity of the alleles are dependent on the variety (varietal effect)
and the microsatellite locus used. The relative quantification of musts can be accomplished by the presented method depending on the presence of overlapping
alleles, and the composition of the varieties and their relative amounts. |
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Keywords: | Vitis vinifera Grape must Microsatellite markers Varietal authentication Quantification |
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