甘蓝型油菜BnERF104超表达增强了转基因拟南芥对核盘菌的抗性 |
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引用本文: | 汪承刚,蔡丽,董彩华,黄军艳,赵美霞,周瑢,刘胜毅.甘蓝型油菜BnERF104超表达增强了转基因拟南芥对核盘菌的抗性[J].中国油料,2011(4):325-330. |
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作者姓名: | 汪承刚 蔡丽 董彩华 黄军艳 赵美霞 周瑢 刘胜毅 |
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作者单位: | 中国农业科学院油料作物研究所,农业部油料作物生物学重点开放实验室,湖北武汉430062 |
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基金项目: | 国家自然科学基金(30900934); 公益性行业(农业)科研专项(201103016) |
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摘 要: | 根据拟南芥乙烯响应因子AtERF104的序列设计保守引物,从甘蓝型油菜品种中双9号中克隆到同源基因BnERF104。序列分析表明BnERF104蛋白含有一个典型的ERF domain保守结构域,属于乙烯响应转录因子ERF家族的成员。甘蓝型油菜用核盘菌(Sclerotinia sclerotiorum)接种6h后,BnERF104基因被诱导显著上调表达。BnERF104基因在转基因拟南芥中的超表达提高了病原相关蛋白(PR)防御素PDF1.2和几丁质酶ChiB基因的表达量,显著增强了对腐生营养型真菌核盘菌的抗性。
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关 键 词: | 乙烯响应转录因子 BnERF104 PR蛋白基因 核盘菌 |
Over-expression of BnERF104 from Brassica napus enhanced transgenic Arabidopsis resistance to Sclerotinia sclerotiorum |
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Authors: | WANG Cheng-gang CAI Li DONG Cai-hua HUANG Jun-yan ZHAO Mei-xia ZHOU Rong LIU Sheng-yi |
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Affiliation: | (Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Oil Crops Biology of the Ministry of Agriculture,Wuhan 430062,China) |
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Abstract: | BnERF104 gene was cloned from Brassica napus cv Zhongshuang 9 using conserved primers designed from AtERF104 gene sequence of Arabidopsis.NCBI-BLAST showed BnERF104 protein had a typical ERF conserved domain,was a member of ETHYLENE-RESPONSE-FACTOR(ERF) family.BnERF104 expression was up-regulated significantly at 6h after S.sclerotiorum inoculation.Over-expression of BnERF104 in transgenic Arabidopsis thaliana activated expressions of pathogenesis-related(PR) gene PDF1.2 and ChiB,and significantly enhanced plant resistance to necrotrophic fungi S.sclerotiorum. |
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Keywords: | ETHYLENE-RESPONSE-FACTOR BnERF104 PR gene Sclerotinia sclerotiorum |
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