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Ferulic acid and EGCG alter the structural characteristics of ovalbumin and its application in mineral loading |
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| Authors: | Zhenzhen Huang Xinxi Yang Qiqi Chen Leqi Chen Siyue Liang Qingzhu Zeng Ruifen Zhang Fei Huang Lihong Dong Dongxiao Su |
| Affiliation: | 1. School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou, Guangdong, 510006 China
These two authors should be considered joint first authors. Contribution: Data curation (equal), ?Investigation (lead), Methodology (equal), Validation (equal), Writing - original draft (supporting);2. School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou, Guangdong, 510006 China These two authors should be considered joint first authors. Contribution: Data curation (equal), ?Investigation (equal), Methodology (equal), Visualization (lead), Writing - original draft (equal);3. School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou, Guangdong, 510006 China Contribution: Data curation (supporting), Validation (equal), Visualization (equal), Writing - review & editing (supporting);4. School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou, Guangdong, 510006 China Contribution: Data curation (supporting), ?Investigation (equal), Methodology (supporting), Validation (supporting);5. School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou, Guangdong, 510006 China Contribution: Conceptualization (supporting), Formal analysis (supporting), Methodology (supporting), Project administration (supporting);6. Sericultural and Agri-Food Research Institute Guangdong Academy of Agricultural Sciences / Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs / Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, 510610 China Contribution: Formal analysis (supporting), Funding acquisition (lead), Resources (supporting);7. Sericultural and Agri-Food Research Institute Guangdong Academy of Agricultural Sciences / Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs / Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, 510610 China Contribution: Formal analysis (supporting), Funding acquisition (supporting), Methodology (supporting), Resources (supporting);8. Sericultural and Agri-Food Research Institute Guangdong Academy of Agricultural Sciences / Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs / Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, 510610 China Contribution: Funding acquisition (supporting), Methodology (supporting), Project administration (supporting), Supervision (supporting);9. School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou, Guangdong, 510006 China |
| Abstract: | This study aims to fabricate mineral-loading nanocarriers using natural materials. The interaction patterns between ovalbumin (OVA) and four water-soluble polyphenols, namely ferulic acid (FA), (-)-Epigallo-catechin 3-gallate (EGCG), gallic acid (GA) and epicatechin (EC), were investigated. Results showed that the optimised conditions for preparing stable OVA–polyphenol complexes are at the OVA–polyphenol ratio of 4:1 at pH 6, under which OVA–FA and OVA–EGCG showed the highest stability and mineral-loading capacity among four OVA–polyphenol complexes. The fluorescence results indicated that the addition of EGCG and FA induced a significant fluorescence quenching to OVA. The interaction between OVA and polyphenols involved hydrogen bonding, hydrophobic interaction and electrostatic interaction. Fourier transform infrared spectroscopy (FTIR) analysis suggested that both FA and EGCG enhanced the stability and orderliness of the structure of OVA. The transmission electron microscopy images also exhibited the spherical structure of OVA after the addition of FA and EGCG. Furthermore, scanning electron microscope–energy dispersive X-ray spectrum results suggested that OVA–FA and OVA–EGCG complexes were better mineral carriers than OVA–GA and OVA–EC. This study may serve as the theoretical support for the promising application of OVA in the fabrication of mineral-loading nanocarriers in functional food and pharmaceutic. |
| Keywords: | interaction mineral loading ovalbumin phenolic stability |
