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Phenolic composition,antioxidant and cytoprotective effects of aqueous-methanol extract from Anneslea fragrans leaves as affected by drying methods |
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| Authors: | Qimin Cui Yudan Wang Wenbing Zhou Shuyue He Meilian Yang Qingwang Xue Yifen Wang Tianrui Zhao Jianxin Cao Afsar Khan Guiguang Cheng |
| Affiliation: | 1. Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming, 650500 China
These authors are co-first authors of the article. They are equally contributed to this paper. Contribution: Data curation (equal), Formal analysis (lead), Software (lead), Writing - original draft (equal);2. Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming, 650500 China National and Local Joint Engineering Research Center for Green Preparation Technology of Biobased Materials, Yunnan Minzu University, Kunming, 650500 China These authors are co-first authors of the article. They are equally contributed to this paper. Contribution: Supervision (lead), Visualization (supporting), Writing - review & editing (supporting);3. Yunnan Tobacco Company, Yuxi Branch, Yuxi, 653100 China Contribution: Funding acquisition (supporting), Resources (supporting);4. Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming, 650500 China Contribution: Data curation (supporting), Formal analysis (supporting);5. Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming, 650500 China Contribution: Conceptualization (supporting), Methodology (lead);6. Department of Chemistry, Liaocheng University, Liaocheng, 252059 China Contribution: Project administration (supporting), Validation (supporting);7. Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223 China Contribution: Methodology (supporting), Software (supporting);8. Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming, 650500 China Contribution: Investigation (supporting), Supervision (supporting);9. Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming, 650500 China Contribution: Funding acquisition (supporting), Resources (supporting);10. Department of Chemistry, COMSATS University Islamabad, Abbottabad Campus, Abbottabad, 22060 Pakistan;11. Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming, 650500 China |
| Abstract: | The present study is aimed to evaluate the effect of three different drying methods, including shade drying (SD), freeze drying (FD) and hot air dying (HAD), on the chemical components, antioxidant and cytoprotective activities of aqueous-methanol extracts from A. fragrans leaves. The total phenolic and flavonoid content were determined by spectroscopic methods, whereas the chemical profile of different extracts was determined by ultra-high-performance liquid chromatography coupled with a tandem mass spectrometry (UHPLC-MS/MS). A total of thirteen compounds were characterised and 9 phenolic compounds have been quantified. Two dihydrochalcone glycosides named confusoside and vacciniifolin were found to be the most abundant phenolic compounds in the SD extract of A. fragrans leaves, which also showed the strongest antioxidant and cytoprotective activities. The results showed that shade drying is an efficient method for preserving the chemical constituents and maintaining the antioxidant and cytoprotective activities of A. fragrans leaves. |
| Keywords: | Anneslea fragrans antioxidant activity cytoprotective activity drying methods flavonoids phenolics |
