| 浏阳豆豉发酵中高产酶活菌株的分离鉴定及酶活性分析 |
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| 引用本文： | 陈怡,刘洋,蒋立文,李跑,伟明,覃业优.浏阳豆豉发酵中高产酶活菌株的分离鉴定及酶活性分析[J].中国酿造,2020,39(8):37. |
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| 作者姓名： | 陈怡 刘洋 蒋立文 李跑 伟明 覃业优 |
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| 作者单位： | （1.湖南农业大学 食品科学技术学院，湖南 长沙 410128；2.食品科学与生物技术湖南省重点实验室，湖南 长沙 410128；3.溢品鲜调味食品厂，湖南 浏阳 410000；4.湖南坛坛香食品科技有限公司，湖南 长沙 410000） |
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| 基金项目： | 国家自然科学基金面上项目（31571819） |
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| 摘 要： | 从浏阳豆豉发酵过程中分离产高酶活菌株，通过形态观察结合分子生物学技术进行鉴定，并对其产蛋白酶、脂肪酶及纤维素酶的活性进行分析。结果表明，分离得到3株菌（编号为000、5132、621）均被鉴定为溜曲霉菌（Aspergillus tamarri）。3株菌的蛋白酶、脂肪酶及纤维素酶的活性测定结果表明，菌株621蛋白酶活性最强，为（207.98±3.20）U/mL；菌株5132的纤维素酶活性最强，为（3.40±1.40）U/mL；菌株000的脂肪酶活性最高，为（90.7±0.64）U/mL。
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| 关 键 词： | 溜曲霉 蛋白酶活力 脂肪酶活力 纤维素酶活力 |
Isolation and identification of strains with high enzyme activity during Liuyang Douchi fermentation and enzyme activity analysis |
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| CHEN Yi,LIU Yang,JIANG Liwen,LI Pao,WEI Ming,QIN Yeyou.Isolation and identification of strains with high enzyme activity during Liuyang Douchi fermentation and enzyme activity analysis[J].China Brewing,2020,39(8):37. |
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| Authors: | CHEN Yi LIU Yang JIANG Liwen LI Pao WEI Ming QIN Yeyou |
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| Affiliation: | (1.College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China; 2.Hunan Provincial Key Laboratory of Food Science and Biotechnology, Changsha 410128, China; 3.Yi-Pin-Xian Seasoning Food Factory, Liuyang 410000, China; 4.Hunan Tantanxiang Food Technology Co., Ltd., Changsha 410000, China) |
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| Abstract: | The strains with high enzyme activity were isolated during Liuyang Douchi fermentation, and identified by morphological observation and molecular biological technique, and the activities of protease, lipase and cellulase were analyzed. The results showed that the 3 isolated strains (No. 000, 5132, 621) were identified as Aspergillus tamarri. The determination results of the protease, lipase and cellulase activities of the 3 isolated strains showed that the protease activity of the strain 621 was the highest of (207.98±3.20) U/ml, the cellulase activity of the strain 5132 was the highest of (3.40±1.40) U/ml, and the lipase activity of the strain 000 was the highest of (90.7±0.64) U/ml. |
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| Keywords: | Aspergillus tamari protease activity lipase activity cellulase activity |
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