| 纤维堆囊菌产埃博霉素B发酵培养基的优化 |
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| 引用本文： | 龚国利,陈松,李慧,曾桥,李楠,宋娟娜.纤维堆囊菌产埃博霉素B发酵培养基的优化[J].中国酿造,2012(10):30-32. |
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| 作者姓名： | 龚国利 陈松 李慧 曾桥 李楠 宋娟娜 |
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| 作者单位： | [1]陕西科技大学生命科学与工程学院,陕西西安710021 [2]陕西科技大学资源与环境学院,陕西西安710021 |
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| 基金项目： | 国家自然科学基金（No.20906058）,陕西省自然科学基金（No.2010JQ4020）,陕两省教育厅自然科学研究专项（No.12JKl023）,默两省科技厅社会发展攻关项目（No.2010K01-200）,陕两科技大学学术骨干培育项日（No.XSG2010009） |
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| 摘 要： | 目的优化埃博霉素B发酵培养基。方法以纤维堆囊菌SoF5—9为供试菌株，通过单因素和止交试验研究了埃博霉素B合成的最佳培养基。结果埃博霉素B最适发酵培养基为糊精0．3%，蔗糖0．07％，葡萄糖0．02％，豆饼粉0．17％，MgSO4·7H200．1‰无水氯化钙0．1％，EDTA．Fe^（3＋）2mL／L，微量元素（TE）0．5mL／L。在该最优条件下，埃博霉素B产量可达到27．5mg／L。结论合理优化发酵培养基组分可以有效提高埃博霉素B的产量。
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| 关 键 词： | 纤维堆囊菌 埃博霉素B 培养基优化 正交试验 |
Optimization of fermentation medium for cpothilone B produced by Sorangium cellulosum |
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| GONG Guoli,CHEN Song,LI Hui,ZENG Qiao,LI Nan,SONG Juanna.Optimization of fermentation medium for cpothilone B produced by Sorangium cellulosum[J].China Brewing,2012(10):30-32. |
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| Authors: | GONG Guoli CHEN Song LI Hui ZENG Qiao LI Nan SONG Juanna |
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| Affiliation: | 1.College of Life Science and engineering, Shanxi University of Science and Technology, Xi＇an 710021, China; 2.College of Resources and Environmen Shanxi University of Science and Technology, Xi＇an 710021, China) |
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| Abstract: | Objective Optimizing epothilone B fermentation medium. Method The medimn of Sorangium cellulosum SOF5-9 for the production of e- pothilone B was optimized by single factor experiment and orthogonal design method. Result The optimum fermentation medium of epothilone B was as followed： dextrin 0.3%, sucrose 0.07%, glucose 0.02%, soybean cake powder 0.17%, MgSO4· 7H20 0.1%, CaCI2 0.1%, EDTA-Fe3. 2mL/L, TE 0.5mL/L. Under the optimal condition, the production of epothilone B reached 27.5mg/L. Conclusion Optimizing fermentation medium can effectively improve the production of epothilone B. |
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| Keywords: | Sorangium Cellulosum epothilone B medium optimization orthogonal experiment |
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