Fluidized-bed drying as a feasible method for dehydration of Enterococcus faecium M74 |
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Authors: | Stefanie Stummer Stefan Toegel Marie-Christin Rabenreither Frank M Unger Michael Wirth Helmut Viernstein Sharareh Salar-Behzadi |
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Affiliation: | 1. Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria;2. Department of Orthopedics, Medical University Vienna, Vienna, Austria |
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Abstract: | The suitability of fluidized-bed technology for the dehydration of probiotic Enterococcus faecium M74 was evaluated. Fluidized-bed drying was processed by layering the microorganisms on spherical pellets. The impact on cell viability of atomizing air pressure, processing temperature and time was investigated. Using 1.5 bar atomizing air pressure, 37 °C processing temperature, and 15 min processing time provided optimal dehydration condition. Changing these values resulted in excessive stress on the cells and affected the cell viability. Next, we compared the impact on cell viability of fluidized-bed drying with that of freeze-drying. Fluidized-bed drying caused more substantial losses of cell viability. However, viability of cells pre-treated with membrane protective agents such as sucrose or skim milk was less affected by fluidized-bed drying than by freeze-drying, resulted in a minor degree of membrane damage after 2 months storage. Comparison of the flow characteristics of freeze-dried cells and fluidized-bed dried cells layered on spherical pellets showed the superior flowability of the latter. |
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Keywords: | Probiotics Dehydration Fluidized-bed drying Cellular properties Solid formulations |
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