电子烟雾化剂丙三醇90天大鼠吸入毒性研究

  目的  了解电子烟雾化剂丙三醇的吸入毒性。  方法  选择120只Wistar大鼠进行90天吸入毒性试验（恢复期28天）。按照大鼠体重随机分为阴性对照组、低剂量组（10 mg/kg）、中剂量组（100 mg/kg）和高剂量组（750 mg/kg），每组动物30只，雌雄各半。试验期间检测大鼠体重和耗食量变化；在染毒期和恢复期结束后，解剖大鼠进行血液学、血生化、尿液等指标检测和肺部支气管灌洗液分析，并对大鼠脏器进行组织病理学检查。  结果  与阴性对照组相比，①体重与耗食量:中剂量组雄性大鼠第3周耗食量降低（P < 0.05），高剂量组雌性大鼠第6和11周耗食量降低（P < 0.05）；②血液学:恢复期中剂量组雄性大鼠，MCH（平均红细胞血红蛋白）水平上升（P < 0.05）；PLT（血小板数）水平下降（P < 0.05）；③血生化:处理组的各项指标均未见显著性差异（P>0.05）④尿液:恢复期低剂量组雄性大鼠的尿比重升高（P < 0.05），⑤脏器重量及系数:染毒期高剂量组雌性大鼠肝脏重量、肾脏重量均低于阴性对照组（P < 0.0）；恢复期中剂量组雄性大鼠肺重量低于阴性对照组（P < 0.01），⑥肺部支气管灌洗液分析:处理组的各项指标均未见显著性差异（P>0.05）⑦组织病理学检查:阴性对照组和高剂量组中大鼠的脏器组织未见明显病变。经分析以上的指标变化未呈现量效关系。  结论  大鼠鼻吸入丙三醇90天，暴露剂量750 mg/kg情况下未对大鼠产生明显的毒性作用。 

Inhalation toxicity testing on Wistar rats exposed to atomized glycerol in 90 days

  Objective  This work aims to understand the inhalation toxicity of glycerin as electronic cigarette atomizing agent.  Methods  In this work, 120 Wistar rats were selected as research objected subjected to 90 days of inhalation toxicity test, with 28 days of recovery. The rats were randomly divided into a negative control group, a low-dose group (10 mg/kg), a medium-dose group (100 mg/kg) and a high-dose group (750 mg/kg) according to their body weight. There were 30 animals in each group, half male and half female. During the experiment, the changes in animal body weight and food consumption were detected. After the exposing period and recovery period, hematology, blood biochemistry, urine and other endpoints were detected, Broncho alveolar lavage fluid was analyzed, and histopathological evaluation of animal organs was conducted.  Results  Compared with the negative control group, ① The male rats in the medium dose group exhibited significantly decreased food consumption at week 3 (P < 0.05), while the female rats in the high dose group exhibited significantly decreased food consumption at week 6 and week 11 (P < 0.05). ② The MCH (mean erythrocyte hemoglobin) level significantly increased in the male rats of medium dose group during recovery (P < 0.05) and the level of PLT (platelet count) was significantly decreased (P < 0.05). ③ There was no significant difference in blood biochemistry among the treatment groups (P>0.05). ④ The urine specific gravity in the low dose group increased during recovery period (P < 0.05). ⑤ The weight of liver and kidney in female rats of the high-dose group were lower than that of the negative control group during exposing period (P < 0.01). During the recovery period, lung weight in male rats of the medium dose group was lower than that of the negative control group (P < 0.01). ⑥ There was no significant difference in broncho alveolar lavage fluid: among the treatment groups (P>0.05). ⑦ No obvious lesions were found in the organs of the negative control group and the high-dose group according to histopathological evaluation. The changes of the above indicators showed no dose-effect relationship.  Conclusion  Therefore, no significant toxic effect was produced on rats after 90 days of inhalation of glycerin at the exposure dose of 750 mg/kg.