| 甘蔗糖蜜发酵培养高铁营养酵母的菌种诱变选育 |
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| 引用本文： | 何海燕,覃拥灵,李楠,梁智群.甘蔗糖蜜发酵培养高铁营养酵母的菌种诱变选育[J].食品研究与开发,2007,28(4):50-53. |
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| 作者姓名： | 何海燕 覃拥灵 李楠 梁智群 |
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| 作者单位： | 1. 广西大学生命科学与技术学院,广西,南宁,530004;河池学院化学与生命科学系,广西,宜州,546300
2. 广西大学生命科学与技术学院,广西,南宁,530004 |
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| 摘 要： | 经过抗性筛选和摇瓶发酵培养，从25株酵母菌中筛选到一株能以甘蔗糖蜜为碳源的生物量较高及富铁能力较强的菌株R4—5。利用^60Co辐射诱变，选育出一株正突变菌株R4—5—27，在同等发酵条件下，生物量达到10．25mg／L，铁含量7．43mg/g，总铁含量为76．16mg/L，比出发菌株提高了1．59。连续10次传代，其产量性状无大变化，表明该菌株遗传性状稳定，极有潜力改良为工业化生产菌种。
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| 关 键 词： | 高铁营养酵母 甘蔗糖蜜 60Co诱变 筛选 |
| 修稿时间： | 2006-09-20 |
SCREENING AND FERMENTING CULTIVATION OF IRON-ENRICHED YEAST WITH SUGARCANE MOLASSES |
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| HE Hai-yan,QIN Yong-ling,LI Nan,LIANG Zhi-Qun.SCREENING AND FERMENTING CULTIVATION OF IRON-ENRICHED YEAST WITH SUGARCANE MOLASSES[J].Food Research and Developent,2007,28(4):50-53. |
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| Authors: | HE Hai-yan QIN Yong-ling LI Nan LIANG Zhi-Qun |
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| Affiliation: | 1. College of Life Science and Technology, Guangxi University, Nanning 530004, Guangxi, China; 2. Departerment of Chemistry and Life Science,Hechi University,Yizhou 546300,Guangxi, China |
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| Abstract: | A high-biomass,iron-enriched yeast strain R4-5 was obtained after gradual screening and fermenting through 25 yeast strain, treated it with ^60Co mutation ,then got a positive mutation yeast strain R4-5-27 that contain more biomass and iron,and this yeast strain can used sugarcane molasses as the culture medium.In the same measure , the biomass was 10.25 mg/L, iron content of the yeast cell was 7.43 mg/g.The total iron content was 76.16 mg/L, 159 % higher than R4-5,and the results of generation experiment show that it is a stabilization and choiceness yeast strain of high biomass and iron. |
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| Keywords: | iron-enriched yeast sugarcane molasses ^60Co mutation screening |
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