超声波辅助酶法优化黄精多糖提取工艺的研究

该文主要以始兴黄精为原料,纯净水为提取溶剂,采用超声波辅助酶法提取黄精多糖,通过单因素试验研究复合酶添加量、酶解时间、酶解温度和料液比等因素对黄精多糖提取率的影响,并对其最佳工艺进行正交试验优化。结果表明,超声波辅助酶法提取黄精多糖的最佳工艺条件为:复合酶添加量6%、酶解温度65℃、酶解时间55 min、料液比1∶30(g/mL),在此工艺条件下得到黄精多糖的提取率为25.63%。

Ultrasonic-assisted Enzymatic Optimization of Extraction Process of Polysaccharides from Polygonatum sibiricum

To used Shixing Polygonatum as the raw material,purified water as the extraction solvent,and used ultrasonic-assisted enzymatic extraction of Polygonatum polysaccharides.Through single factor experiments,the effects of compound enzyme addition,digestion time,digestion temperature and material-liquid ratio on the extraction rate of Polygonatum polysaccharides were studied,and optimize the best process by orthogonal experiments.The results showed that the optimal process conditions of ultrasonic-assisted enzymatic extraction of Polygonatum polysaccharides were 6%compound enzyme addition,enzymolysis temperature 65℃,enzymolysis at a time of 55 min and a material-to-liquid ratio of 1∶30(g/mL),the extraction rate of Polygonatum polysaccharide under this process condition was 25.63%.