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| 抗草甘膦转基因大豆加工品的PCR检测研究 | |
| 引用本文： | 黄亚东,白卫滨,孙建霞,柳忠玉,赵文,苏志坚,程树军,李丹宁.抗草甘膦转基因大豆加工品的PCR检测研究[J].食品研究与开发,2006,27(3):119-122. |
| 作者姓名： | 黄亚东 白卫滨 孙建霞 柳忠玉 赵文 苏志坚 程树军 李丹宁 |
| 作者单位： | 1. 暨南大学生物医药开发中心,广东,广州,510630 2. 暨南大学生物医药开发中心,广东,广州,510630;山东省农业管理干部学院,山东,济南,250100 3. 山东省农业管理干部学院,山东,济南,250100 4. 广州进出口检验检疫局,广东,广州,510623 |
| 摘 要： | 以进口抗草甘膦转基因豆粕、豆粉和中国脱脂大豆为材料,利用设计的特殊引物,建立了PCR技术定性检测抗草甘膦转基因大豆加工品的方法,成功检测出进口抗草甘膦转基因豆粕和豆粉的内源参照基因Lectin、CaMV35S/CTP of EPSPS边界序列和NOS终止子,国产脱脂大豆仅检出内源参照基因Lectin,确定了此PCR技术检测抗草甘膦转基因大豆的灵敏度为0.1%,稳定性良好. |
| 关 键 词： | 转基因豆粕 豆粉 脱脂大豆 PCR检测 |
| 收稿时间： | 2005-11-07 |
| 修稿时间： | 2005年11月7日 |
STUDY ON THE DETECTION OF GENETICALLY MODIFIED SOYBEAN PRODUCTS BY POLY MERASE CHAIN REACTION |
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| HUANG Ya-dong,BAI Wei-bin,SUN Jian-xia,LIU Zhong-yu,ZHAO Wen,SU Zhi-jian,CHENG Shu-jun,LI Dan-ning.STUDY ON THE DETECTION OF GENETICALLY MODIFIED SOYBEAN PRODUCTS BY POLY MERASE CHAIN REACTION[J].Food Research and Developent,2006,27(3):119-122. | |
| Authors: | HUANG Ya-dong BAI Wei-bin SUN Jian-xia LIU Zhong-yu ZHAO Wen SU Zhi-jian CHENG Shu-jun LI Dan-ning |
| Abstract: | Using the glyphosate-resistant Genetically Modified Soybean Meal, soybean powder and Chinese degreased soybean as testing materials, and with specially designed primer, the research takes the Polymerase Chain Reaction (PCR) technique qualitative detecting method of glyphosate-resistant GMS. The outcome is that we successfully detect the existence of the endogenesis gene Lectin, CaMV35S/CTP of EPSPS boundary sequence and NOS terminator in the imported the glyphosate-resistant Genetically Modified Soybean Meal and Soybean powder, on the other hand, no other gene but the endogenesis gene Lectin has been found in Chinese soybean samples. The research also succeeds in settling the 0.5% precision degree of the glyphosate-resistant GMS which ensures its stable property. |
| Keywords: | Genetically Modified Soybean Meal soybean powder degreased soybean PCR detection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
