| 环介导等温扩增技术检测食品中铜绿假单胞菌 |
| |
| 引用本文： | 刘伟,侯丽萍,赵良娟,曲鹏,郑文杰,张宏伟.环介导等温扩增技术检测食品中铜绿假单胞菌[J].食品研究与开发,2012,33(5):140-142. |
| |
| 作者姓名： | 刘伟 侯丽萍 赵良娟 曲鹏 郑文杰 张宏伟 |
| |
| 作者单位： | 天津出入境检验检疫局,天津,300201 |
| |
| 摘 要： | 建立食品中铜绿假单胞菌环介导等温扩增(LAMP)检测方法。利用铜绿假单胞菌外毒素A(PEA)基因序列,设计3对铜绿假单胞菌LAMP检测特异性引物,用36株铜绿假单胞菌,14株近源菌验证方法的特异性。建立的LAMP方法特异性好,灵敏度达到2.2 cfu/100 g~3.5 cfu/100 g。建立的食品中铜绿假单胞菌LAMP检测方法特异性好,灵敏度高,适用于的检测食品中的铜绿假单胞菌。
|
| 关 键 词： | 铜绿假单胞菌 PEA基因 环介导等温扩增技术(LAMP) 食品 |
Development of Loop-mediated Isothermal Amplification(LAMP) Detection of Pseudomonas Aeruginosa in Food |
| |
| LIU Wei
,
HOU Li-ping
,
ZHAO Liang-juan
,
QU Peng
,
ZHENG Wen-jie
,
ZHANG Hong-wei.Development of Loop-mediated Isothermal Amplification(LAMP) Detection of Pseudomonas Aeruginosa in Food[J].Food Research and Developent,2012,33(5):140-142. |
| |
| Authors: | LIU Wei HOU Li-ping ZHAO Liang-juan QU Peng ZHENG Wen-jie ZHANG Hong-wei |
| |
| Affiliation: | (Tianjin Entry-exit Inspection and Quarantine Bureau,Tianjin 300201,China) |
| |
| Abstract: | A novel LAMP detection method of pseudomonas aeruginosa in food was developed in present study.The LAMP detection targeted the PEA gene sequence with 3 pairs of specific primers.The detection was evaluated by 36 strains of pseudomonas aeruginosa and 14 strains of pseudomonas.The sensitivity of LAMP detection was 2.2 cfu-3.5 cfu of pseudomonas aeruginosa per 100 g food.It’s an economic method for extend in food industry. |
| |
| Keywords: | Pseudomonas Aeruginosa PEA gene loop-mediated isothermal amplification(LAMP) food |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
