Antityrosinase and antimicrobial activities of 2-phenylethanol, 2-phenylacetaldehyde and 2-phenylacetic acid |
| |
| Authors: | Yu-Jing Zhu Han-Tao Zhou Yong-Hua Hu Jian-Yang Tang Ming-Xing Su Yun-Ji Guo Qing-Xi Chen Bo Liu |
| |
| Affiliation: | 1. Agricultural Bio-resources Institute, Fujian Academy of Agricultural Sciences, Fuzhou, China;2. Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, China;3. Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen, China |
| |
| Abstract: | The inhibition kinetics of 2-phenylethanol, 2-phenylacetaldehyde and 2-phenylacetic acid on the enzyme activity of mushroom tyrosinase have been investigated. The results showed that these aromatic compounds can lead to reversible inhibition of the enzyme; furthermore, 2-phenylacetaldehyde and 2-phenylacetic acid are uncompetitive inhibitors and 2-phenylethanol is a mixed-type inhibitor. The inhibition constants have been determined and the inhibiting ability was: 2-phenylacetaldehyde > 2-phenylacetic acid > 2-phenylethanol, indicating that the functional group on the benzene ring group played an important role in the inhibition of the enzyme. In addition, 2-phenylacetic acid and 2-phenylethanol were found to have effective antibacterial activities, and 2-phenylacetic acid was more effective against Escherichia coli and Ralstonia solanacearum than 2-phenylethanol, but 2-phenylacetaldehyde lacked of antibacterial activity. |
| |
| Keywords: | l-DOPA l-3 4-dihydroxyphenylalanine DMSO dimethylsulfoxide IC50 the inhibitor concentrations leading to 50% activity lost MIC the minimum inhibitory concentration MBC the minimum bactericidal concentration KI the equilibrium constant of the inhibitor combining with the free enzyme KIS the equilibrium constant of the inhibitor combining with the enzyme&ndash substrate complex |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
